Enterobacteriaceae Flashcards

1
Q

H2S (+)

A

SPACE:
Salmonella
Proteus
Arizonae
Citrobacter freundii
Edwardsiella tarda

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2
Q

How to differentiate PPM?

A

Proteus:
• Swarming
• H2S +
• P. mirabilis (Indole [-])
• P. vulgaris (Indole [+])

Providencia: Citrate (+)
Morganella: ODC (+)

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3
Q

Differentiate E. coli vs. Edwardsiella

A

Edwardsiella is:
• H2S + w/ gas
• NLF (K/A)

*E. coli is H2S -; they both have same IMVIC of ++—

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4
Q

Differentiate Y. pestis from enterocolitica

A

Urease test
• Pestis is -
• Enterocolitica is +; slow

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5
Q

Cauliflower appearance of colonies

A

• 48 hrs in SBA: Y. pestis
• 4-8 months in LJ: M. tb

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6
Q

Most common cause of death associated w/ bacterial contamination of packed RBCs

A

Y. enterocolitica

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7
Q

BULL!
* Bull’s eye colonies on CIN: ?
* Bull’s eye rash (erythema chronicum migrans): ?
* Bull’s eye amoeba: ?
* Bull’s neck infection: ?
* Buffalo hump: ?

A
  • Bull’s eye colonies on CIN: Y. enterocolitica
  • Bull’s eye rash (erythema chronicum migrans): Borrelia burgderfori
  • Bull’s eye amoeba: E. histolytica
  • Bull’s neck infection: C. diphtheriae
  • Buffalo hump: Cushing’s syndrome
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8
Q

Fish eye colonies on EMB

A

Enterobacter

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9
Q

Tests to detect H2S positive

A

TXL, H2S

  • TSI
  • SSA
  • XLD
  • HEA
  • SIM
  • LIA
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10
Q

Neutral red indicator

A

SSMac

*SSA
*MAC

A: Red
K: Yellow
Fermenters: Pink-red colonies
NF: Colorless colonies

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11
Q

Triple LOA (+): ?
Triple LOA (-): ?

A

Triple LOA (+): Plesiomonas
Triple LOA (-): Pantoea

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12
Q

Indole (+)

A

MAY PEKPEC PO?

  • Morganella
  • P. vulgaris
  • E. coli
  • K. oxytoca
  • Providencia
  • E. tarda
  • C. koseri
  • Plesiomonas
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13
Q

VP (+)

A

KEESH

*Klebsiella
*Ewingella
*Enterobacter
*Serratia
*Hafnia

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14
Q

Citrate (+)

A

Pero Sa CHEEKS
*Providencia
*Serratia
*Citrobacter
*Hafnia
*Ewingella
*Enterobacter
*Klebsiella
*Salmonella enteritidis

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15
Q

Tests with same result: (+) and (-)

A

MEEC

*Malonate
*Acetate
*Acetamide
*Citrate

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16
Q

Rapid urease (+)

A

TPUNCH

*T. mentagrophytes
*PPM (except P. stuartii)
*Ureaplasma
*Nocardia
*C. perfringens
*H. pylori

17
Q

Slow urease (+)

A

CKEYS

*Citrobacter
*Klebsiella
*Enterobacter gergoviae
*Y. enterocolitica
*Serratia

18
Q

Differentiate Y. enterocolitica vs. Y. pestis

A
  • Urease test
  • Motility (pestis is non-motile)
19
Q

Differentiate Y. enterocolitica vs. Aeromonas

A

*Urease (Y: slow; A: non)
*Oxidase (Y: CPON; A: CPOP)
*Freshwater fish (?)

20
Q

Gold standard for quorum sensing

A

Chromobacterium violaceum

21
Q

Key tests to differentiate Aeromonas & Plesiomonas vs. Vibrio

A
  • Sodium requirement
  • Mannitol fermentation