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histoopaathh > Infiltration/Impregnation > Flashcards

Infiltration/Impregnation Flashcards

1
Q

Purpose of infiltration

A

To remove clearing agent from tissues
To fill cavities and tissue spaces to give firm consistency for easy cutting

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1
Q

Purpose of infiltration

A

To remove clearing agent from tissues
To fill cavities and tissue spaces to give firm consistency for easy cutting

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2
Q

Infiltration Methods:

A
  1. Manual
  2. Automatic
  3. Vacuum
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3
Q

Paraffin wax substitute: (4)

A
  1. Paraplast
  2. Bioloid
  3. Carbowax
  4. Ester Wax
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4
Q

Infiltration under negative atmospheric pressure inside the oven

A

Vacuum

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5
Q

Most rapid method as it subject tissues to a very high temperature
that facilitates removal of transition solvents and prolongs life of wax
by preventing solvent contamination

A

Vacuum

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6
Q

Uses autotechnicon which requires 3-4 changes of wax

A

Automatic

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7
Q

Temperature range for manual infiltration

A

55-60°C

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8
Q

Approximate temperature when using manual infiltration

A

2-5°C higher than the wax melting point

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9
Q

Recommended for bones

A

Paraplast

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10
Q

Melting point of Paraplast

A

56-57°C

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11
Q

Substitute for paraffin wax used for eye specimens

A

Bioloid

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12
Q

Paraffin substitute that is water soluble

A

Carbowax

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13
Q

What is carbowax?

A

A polyethylene glycol that has 18 or more carbon atoms

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14
Q

Appears solid at room temperature

A

Carbowax

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15
Q

Recommended for enzyme histochemistry

A

Carbowax

16
Q

Melting point of Ester Wax

A

46-48°C

17
Q

Substitute for paraffin wax that requires heavy duty microtome

A

Ester wax

18
Q

Recommended for specimens with large and hollow cavities that tends to collapse

A

Celloidin/Collodion infiltration

19
Q

A very slow process which involves submerging of tissue in different concentration

A

Celloidin infiltration

20
Q

Recommended for whole eye specimens

A

Dry celloidin

21
Q

Dry celloidin require the use of what mixture to store blocks

A

Gilson’s mixture

22
Q

Celloidin infiltration method for bones, brain, & teeth specimens

A

Wet celloidin

23
Q

Wet celloidin require the use of which agent to store blocks?

A

70-80% alcohol

24
Q

More preferred than usual celloidin as it produces harder blocks and allows cutting of thinner section

A

Low Viscosity Nitrocellulose (LVN)

25
Q

Soluble in equal concentration of ether and alcohol

A

Low Viscosity Nitrocellulose (LVN)

26
Q

Has a lower viscosity which causes higher concentration and can
still penetrate tissues rapidly

A

Low Viscosity Nitrocellulose (LVN)

27
Q

Disadvantage of Low Viscosity Nitrocellulose (LVN) and its remedy

A

Tissues have tendency to crack

Remedy: Add plasticizers (Oleum Ricini & Castor oil)

28
Q

Infiltration method for Enzyme and Histochemical studies

A

Gelatin infiltration

29
Q

This is only done when dehydration is to be avoided

A

Gelatin infiltration

30
Q

In gelatin infiltration method, this can be used to prevent growth of molds

A

Phenol

31
Q

Gilson mixture components

A

Chloroform + Cedar wood oil

histoopaathh (7 decks)

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