ELISA Background Info Flashcards
1
Q
ELISA stands for…
A
Enzyme Linked ImmunoSorbent Assays
in Veterinary Diagnostics
2
Q
What is ELISA
A
technique that uses antibodies and colour changes to identify or quantify a substance.
3
Q
Applications of ELISA
A
•Specific protein/peptides
–CRP, troponin, insulin,
•Infectious disease
–Virus detection
–Specific antibody responses; Serology, antibody response to pathogen
•Allergy testing
•Low MW biochemicals of low concentration
–Hormones
•Thyroxine, cortisol, progesterone
–Drugs (therapeutic and illegal)
4
Q
Name 3 endocrine assays
A
Thyroid function tests
Hyperadrenocorticism (Cushing’s syndrome)
Progesterone assay- pregnancy detection
5
Q
Immunoassays use what for measurement?
A
Sensitivity & specificity
