Haemophilus

Question 1

Haemophilus

Answer 1

-gram negative coccobacillus
-non motile
-normal flora of upper respiratory tract

Question 2

Requires X factor? (hemin) +
Requires V factor? (NAD) +
B-hemolysis of horse blood? =
Ability to convert ALA to Protoporphyrin? =

Answer 2

H. influenza
-type B most common cause of meningitis in children
-type aegyptius causes pink eye

Question 3

Requires X factor? (hemin) +
Requires V factor? (NAD) +
B-hemolysis of horse blood? +
Ability to convert ALA to Protoporphyrin? =

Answer 3

H. haemolyticus

Question 4

Requires X factor? (hemin)=
Requires V factor? (NAD) +
B-hemolysis of horse blood? =
Ability to convert ALA to Protoporphyrin? +

Answer 4

H. parainfluenzae

Question 5

Requires X factor? (hemin) =
Requires V factor? (NAD) =
B-hemolysis of horse blood? =
Ability to convert ALA to Protoporphyrin? +

Answer 5

H. aphrophilus
-associated with endocarditis
-HACEK member

Question 6

Requires X factor? (hemin) +
Requires V factor? (NAD) =
B-hemolysis of horse blood? =
Ability to convert ALA to Protoporphyrin? =

Answer 6

H. ducreyi
-STD that results in genital ulcers and swollen lymph nodes

Question 7

Ability to convert ALA to Protoporphyrin

Answer 7

Confirmatory test to identity Haemophilus species that don’t require X factor to grow. They possess the ability to synthesize it on their own.

Question 8

H. influenza
H. haemolyticus
H. parainfluenzae
H. aphrophilus
H. ducreyi

Answer 8

-all grow on choc agar (supplies X and
V factors)
-grow on horse or rabbit agar
-DONT grow on sheep blood because it contains NADase, which inactivates NAD