Fixatives Flashcards
When is glutaraldehyde used?
for Electron Microscopy
True or False. Glutaraldehyde needs to be diluted also since we cannot use it concentrated.
True
Percentage of glutaraldehyde used for small tissue fragments or needle biopsies.
2.5% solution
Percentage of glutaraldehyde used for large tissues less than 4mm thick.
4% solution
Type of formaldehyde fixative that preserve CNS tissue and post mortem specimens.
10% formol saline
This is a diluted form of formalin which uses NSS.
10% formol saline
Type of formaldehyde fixative for tissues with iron pigments.
10%NeutralBufferedformalin
What is the difference between 10%NeutralBufferedformalin and 10% formalin?
Same with 10% formalin EXCEPT that sodium phosphate is added as a buffer
Type of formaldehyde fixative that is used for preserving lipids and neutral phospholipids.
Formol corrosive (Formol sublimate)
Composition of Formol corrosive (Formol sublimate).
Formalin + Mercuric Chloride
Type of formaldehyde fixative, aside from 10% formalin, used for sputum specimen and for microincineration technique.
Alcoholic formalin (Gendres)
Composition of Alcoholic formalin (Gendres).
Ethyl alcohol + Glacial acetic acid + formalin
Regarded as the smallest aldehyde but rapid/fast acting.
Glyoxal
Enumerate the ALCOHOLIC FIXATIVES
Ethanol
Methanol
Isopropyl (IPA)
Carnoy’s
Newcomer’s
What are the 2 fixatives that are used to preserve brain tissue for diagnosis of rabies.
Acetone and carnoy`s fluid