Decalcifying Agent Flashcards
Most potent anticoagulant but weak decalcifying agent.
EDTA
Binds with calcium to form weakly dissociated complexes.
EDTA
An excellent bone decalcifier for EM and Enzyme histochemistry.
EDTA
What is the disadvantage of EDTA.
It can inactivate alkaline phosphatase
What is the remedy for inactivation of alkaline phosphatase by EDTA.
Magnesium Chloride
Decalcification method that involves attraction of positively charged calcium ions with negatively charged electrode.
Electrolytic Method
Most rapid decalcification method.
Electrolytic Method
Application of electric current in a specimen submerged in acid.
Electrolytic Method
Decalcifying acid used for surface decalcification of blocks.
Von Ebner’s
Composition of Von Ebner’s.
HCl + saline (NSS)
Both a decalcifying agent and a tissue softener.
Pereyni’s Fluid
Most commonly used decalcifying agent especially recommended for urgent biopsies, for needle and small biopsies.
5-10% Nitric Acid
Most rapid nitric containing decalcifying agent.
Phloroglucin Nitric Acid
