Coronaviruses Flashcards
1
Q
Genome
A
(+)ssRNA
2
Q
Structure
A
Helical and enveloped
3
Q
VAP
A
Spike (S)
4
Q
Host receptor
A
ACE2
5
Q
Fusion peptide
A
Exposed by cleavage of Spike protein by TMPRSS2
6
Q
Envelope protein (E)
A
Assembly, membrane channel activity
7
Q
Membrane glycoprotein (M)
A
Assembly
8
Q
Nucleocapsid (N)
A
Coats the genome
9
Q
What is a critical step for entry
A
Processing of Spike by TMPRSS2 to reveal fusion peptide
10
Q
Life cycle
A
- Binding and fusion with Spike-ACE2 and fusion protein
- Initial translation of orf1 to make double membrane vesicles
- Transcription on virus induced membranes
- Transcription of mRNAs
- Translation of structural proteins
- Genome replication
- Budding of RNA/N into an ER vesicle
- Furin matures then it’s released
11
Q
What does the -1 ribosomal Frameshift do?
A
Causes polymerase to fall off
12
Q
PL2pro
A
Responsible for first few cleavages
13
Q
3CLpro
A
Responsible for many last cleavages
14
Q
RNA EXOnuclease
A
Lowers mutation rate by proofreading
15
Q
Remdesivir
A
Inhibits RNAP by preventing elongation