Methanosarcina barkeri Flashcards
1
Q
What’s unusual about M. barkeri?
A
auto fluorescent coenzyme F420
nickel containing coenzyme F430
22nd amino acid pyrrolysine (O, Pyl)
2
Q
What enzyme does M. barkeri use for methanogenesis?
A
Methyl-coenzymeM reductase
3
Q
What energy sources can M. barkeri use?
A
CO2/H2 or acetate
4
Q
What autotrophic CO2 fixation pathway does it use?
A
A modified Wood-Ljungdahl pathway (reductive aceytl-CoA pathway)
5
Q
What type of organism is M. barkeri?
A
Archaea
6
Q
What does methyl-CoM reductase do?
A
Turns methanol, methylamides, and methylsulfides into methane
7
Q
What’s weird about pyrrolysine?
A
It’s encoded by a stop codon in the mRNA for methylamine transferases
Has its own tRNA that is charged by its own aminoacyl tRNA synthetase
