Bacterial Identification Flashcards
1
Q
Staphylococcos aureus Identification
- Arrangement:
- O2 Requirement:
- Hemolysis:
- Glucose utilization:
- Bacitracin:
- Furazolidone:
- Lysostaphin
- Microdase:
- Catalase:
- Coagulase:
- DNAse:
- Colonies:
A
- Arrangement: Grape-like in clusters
- O2 Requirement: Facultative anaerobe
- Hemolysis: Beta hemolysis
- Glucose utilization: Fermentation
- Bacitracin: Resistant
- Furazolidone: Susceptible
- Lysostaphin: Susceptible
- Microdase: Negative
- Catalase: Positive
- Coagulase: Positive
- DNAse: Positive
- Colonies: Golden yellow colonies
2
Q
Micrococci Identification
- Arrangement:
- O2 Requirement:
- Hemolysis:
- Glucose utilization:
- Bacitracin:
- Furazolidone:
- Lysostaphin
- Microdase:
- Catalase:
- Coagulase:
A
- Arrangement: Tetrads/Packets
- O2 Requirement: Obligate aerobe
- Hemolysis: Non-hemolytic
- Glucose utilization: Oxidation (open tube +)
- Bacitracin: Susceptible
- Furazolidone: Resistant
- Lysostaphin: Restant
- Microdase: Positive
- Catalase: Positive
- Coagulase: Negative
3
Q
S. epidermidis Identification
- Catalase:
- Coagulase:
- Novobiocin:
- BAP:
A
- Catalase: Positive
- Coagulase: Negative
- Novobiocin: Susceptible (>16 mm)
- BAP: Sticky white
4
Q
S. saprophyticus Identification
- Catalase:
- Coagulase:
- Novobiocin:
- BAP:
A
- Catalase: Positive
- Coagulase: Negative
- Novobiocin: Resistant (<16 mm)
- BAP: Large yellow/orange/white
5
Q
Identification of Group A - S. pyogenes
- Bacitracin:
- SXT:
- PYR Test:
- CAMP Test:
- Hippurate Test:
A
- Bacitracin: Susceptible
- SXT: Resistant
- PYR Test: Positive
- CAMP Test: No enhanced hemolysis
- Hippurate Test: Negative
6
Q
Identification of Group B - S. agalactiae
- Bacitracin:
- SXT:
- PYR Test:
- CAMP Test:
- Hippurate Test:
- Bile Esculin:
- Growth in 6.5%:
A
- Bacitracin: Resistant
- SXT: Resistant
- PYR Test: Negative
- CAMP Test: Arrowhead hemolysis
- Hippurate Test: Positive
- Bile Esculin: Negative
- Growth in 6.5%: Positive
7
Q
Identification of S. pneumoniae
- Optochin:
- Bile Solubility:
- Inulin Fermentation:
- Animal inoculation:
- Neufeld quellung’s test:
A
- Optochin: Susceptible
- Bile Solubility: Soluble
- Inulin Fermentation: Positive
- Animal inoculation: Positive
- Neufeld quellung’s test: Capsulaw swelling
8
Q
Identification of Viridans Streptococci
- Optochin:
- Bile Solubility:
- Growth in 6.5% NaCl:
- Bile esculin:
- Inulin Fermentation:
- Animal inoculation:
- Neufeld quellung’s test:
A
- Optochin: Resistant
- Bile Solubility: Insoluble
- Growth in 6.5% NaCl: Positive
- Bile esculin: Negative
- Inulin Fermentation: Negative
- Animal inoculation: Negative
- Neufeld quellung’s test: Negative
9
Q
Identification of E. coli
- MAC:
- EMB:
- TSIA:
- IMVC:
- LOA:
- Motility:
- H2S:
- Urease:
- PAD:
A
- MAC: flat, dry with sorrounding dark pink area precipitated with bile salts
- EMB: Green metallic sheen
- TSIA: A/A
- IMVC: ++00
- LOA: ++0
- Motility: Positive
- H2S: Negative
- Urease: Negative
- PAD: Negative
10
Q
Identification of K. pneumoniae
- MAC:
- TSIA:
- IMVC:
- Motility:
- LOA:
- H2S:
- PAD:
- Urease:
A
- MAC: Mucoid colonies
- TSIA: A/A
- IMVC: –++
- Motility: Nonmotile
- LOA: +00
- H2S: Negative
- PAD: Negative
- Urease: Positive
11
Q
Identification of K. oxytoca
- MAC:
- TSIA:
- IMVC:
- Motility:
- LOA:
- H2S:
- PAD:
- Urease:
A
- MAC: Mucoid colonies
- TSIA: A/A
- IMVC: +-++
- Motility: Nonmotile
- LOA: +00
- H2S: Negative
- PAD: Negative
- Urease: Positive
