7-8: DNA Mutation and Repair Flashcards

1
Q

In an auxotrophic yeast mutant requiring adenine, which stage of the adenine biosynthesis pathway would have to be mutated to produce a red pigment?

A

Enzyme 6

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2
Q

What is the significance of the RAD9 gene?

A

Cells that have a deletion or mutation in Rad9 (i.e. rad9 mutants) fail to arrest at G2, even if they have sustained significant damage to their DNA

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3
Q

How can UV affect DNA?

A

UV can be absorbed by aromatic rings of purine/pyrimidine bases, forming pyrimidine dimers, thus disrupting the double helix of DNA and preventing replication

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