7-8: DNA Mutation and Repair Flashcards
1
Q
In an auxotrophic yeast mutant requiring adenine, which stage of the adenine biosynthesis pathway would have to be mutated to produce a red pigment?
A
Enzyme 6
2
Q
What is the significance of the RAD9 gene?
A
Cells that have a deletion or mutation in Rad9 (i.e. rad9 mutants) fail to arrest at G2, even if they have sustained significant damage to their DNA
3
Q
How can UV affect DNA?
A
UV can be absorbed by aromatic rings of purine/pyrimidine bases, forming pyrimidine dimers, thus disrupting the double helix of DNA and preventing replication