Equipment Lists

Question 1

Name the equipment required for the Cytochrome c experiment

Answer 1

Pipettes + Tips
Spectrophotometer
Cuvettes
10 ml + 20 ml measuring cylinders
Sodium dithionate crystals
C-25 ion exchange column (CM)

Diluted heart extract
25 mM phosphate running buffer
25 mM phosphate elution buffer (NaCl)

Question 2

Name the equipment required for the Rapid Reaction experiment

Answer 2

Pipettes + Tips
Spectrophotometer
Cuvettes
Stirring rod
Ice

YADH
1M ethanol
150 mM sodium pyrophosphate buffer
10 mM nAD+

Question 3

Name the equipment required for the Transformation experiment

Answer 3

Innoculation Loops
Pipettes + Tips
Bunsen Burner
Microfuge Tubes
Ice
Sterile Spreaders
Vortex Machine
Agar Plates (w/ antibiotics)
CaCl2 solution

pGLO plasmid
Amp-sensitive E. coli
LB Broth

Question 4

Name the equipment required for the Conjugation Experiment

Answer 4

Agar Plates (w/ antibiotics)
LB Broth
Pipettes + Tips
Microfuge Tubes
Sterile Loops
Bunsen Burner
Universal Tubes
Forceps
Vortex
Virkon Detergent
Microfuge
Matches
Gloves

Question 5

Name the equipment required for the SDS-PAGE experiment

Answer 5

Scalpel
Fish Samples
Microfuge Tubes
Laemmli Buffer
Forceps
SDS-PAGE kit
Pipettes + Tips

Question 6

Name the equipment required for the PCR Experiment

Answer 6

Agarose Gel Electrophoresis
Microwave
Bench Centrifuge
Pipettes + Tips
Universal Tubes
Microfuge/Eppendorf Tubes
Thermocycler
Ethidium Bromide
SafeView
Light Microscope

Question 7

Name the equipment required for the Mutation + Repair experiment

Answer 7

Haemocytometer
Pipettes + Tips
Agar plates
UV chamber
Microscope
Eppendorf Tubes
Universal Tubes
Sterile spreader