14 - Membrane Contact sites Flashcards
What is the rough endoplasmic reticulum responsible for?
Protein synthesis
What is the smooth endoplasmic reticulum responsible for?
Its a site of lipid synthesis and it also has roles in calcium storage.
What do advances in electron microscopy allow us to do?
Understand membrane structure and function better
What dos CLEM (correlative light and electron microscopy) allow us to do?
- Allows us to combine light microscopy with high resolution Electron microscopy.
- Allows the localisation of fluorescently labelled protein to the membrane contact sites.
What is the structure of ER contact sites?
- The ribosomes are excluded.
- Endoplasmic reticulum contacts can be short or long lived.
What is OSBP (Oxysterol binding protein)?
A tether and a lipid transfer protein.
When is there a lot of calcium mobilisation?
At muscle contraction
What is a calcium sensor in the ER?
STIM 1
What do lipid transfer proteins use to promote lipid transfer?
The concentration gradients of lipid.
What is Nieman Pick disease?
An accumulation of lipids in the spleen, liver, lungs bone marrow and the brain.
What other functions do membrane contact sites help with?
They participate in cell signalling and help with the regulation of transport of epidermal growth factor receptor (EGFR) to multivesicular bodies.
What is EGFR phosphorylated by?
PTP1B (protein tyrosine 1B)
