Fine Need Aspiration

Question 1

study of cellular samples obtained from organs that do not shed cells spontaneously, such as breast, thyroid, lymph nodes, liver, lungs, skin, soft tissues and bones

Answer 1

Fine Needle Aspiration

Question 2

organs that do not shed cells spontaneously

Answer 2

breast, thyroid, lymph nodes, liver, lungs, skin, soft tissues and bones

Question 3

T/F.

FNA is useful in lesions that are easily palpable

Answer 3

True

Question 4

FNA basic technique uses

Answer 4

25-gauge needle
10-ml syringe.

Question 5

Requires larger bore needle and increased suction may be necessary

Answer 5

Tissues composed of MESENCHYMAL CELLS

Question 6

Mesenchymal cells adhere to each other and do not _____

Answer 6

Exfoliate easily

Question 7

FNA that is usually done by Clinicians and pathology

Answer 7

FNA of Superficial masses

• breast
• thyroid
• peripheral lymph nodes

Question 8

Deeply seated lesions are

Answer 8

• lung
• mediastinum
• abdominal organs (liver, pancreas, etc.)
• retroperitoneal organs (kidney, adrenal, lymph nodes

Question 9

Deeply seated lesions are performed under

Answer 9

CT scan (Laparoscopy)
Ultrasound (Sonography)

Question 10

sample should be air dried as quickly as possible to ___________

Answer 10

Reduce the effects of shrinkage

Question 11

FNA slide preparation:

most diagnostic material for cytologic evaluation

Answer 11

Few drops from Solid lesion

Question 12

FNA slide preparation:

Specimen is bloody leads to

Answer 12

• Diluted cells
• Hard to find on direct smear

Question 13

FNA slide preparation:

Maximum slides to be prepared

Answer 13

4 slides

Question 14

FNA slide preparation:

How many drops per slide

Answer 14

1-2 drops each slide

Question 15

FNA slide preparation:

Smearing technique used

Answer 15

Slide-pull technique

Question 16

FNA slide preparation:

Rinse the needle in a preservative solution such as _______

Answer 16

Saccomano fluid

Question 17

FNA slide preparation:

Ideal aspirate is ______

Answer 17

creamy consistency with numerous cells suspended in a small amount of tissue fluid

Question 18

FNA slide preparation:

In lymph node aspiration, _______ can be prepared in addition to smear.

Answer 18

Cell suspension

Question 19

FNA slide preparation:

T/F.

Smears obtained by FNA are fixed based on the requirements of the stain to be used.

Answer 19

True

Question 20

FNA slide preparation:

Colloid, mucin and smears to be stained with ____

Answer 20

Hematoxylin stain (May-Grunwald-Giemsa)

Question 21

FNA slide preparation:

useful in preliminary assessment of adequacy of the sample

Answer 21

Rapid stain (Diff Quik 2-3 mins)

Question 22

FNA slide preparation:

Smears to be stained by _________or ______ should be rapidly fixed in alcohol (wet fixation)

Answer 22

Papanicolaou (Pap)

hematoxylin and eosin (H&E)

Question 23

FNA slide preparation:

Specimen stained by Papanicolaou (Pap) or H&E exhibits

Answer 23

Nuclear details

Better identification of malignant cells

Question 24

FNA slide preparation:

In PAP and H&E if the specimen has artefacts it leads to

Answer 24

• more eosinophilic cytoplasm
• details appear fuzzy

Question 25

FNA slide preparation:

smeared by two-slide pull method

Answer 25

Transbronchial Fine needle Aspiration (FNA)