MISCELLANEOUS TESTS

Question 1

MISCELLANEOUS TESTS FOR MOLD IDENTIFICATION (5)

Answer 1

1. Hair Perforation Test
2. Urease
3. Thiamine Requirement
4. Trichophyton Agars
5. Growth on Rice Grains

Question 2

Hair Perforation Test
➢ sterile ______ hair fragments + sterile ____ + sterile _____ extract
➢ examined ___________ and at ________ for a month
➢ T. mentagrophytes - _______
➢ T. tonsurans – _______

Answer 2

➢ sterile 5-10mm hair fragments + sterile H2O + sterile 10% yeast extract
➢ examined microscopically and at weekly intervals for a month
➢ T. mentagrophytes - ectothrix
➢ T. tonsurans – endothrix

Question 3

Urease
(+) color: _______– (2 positive organisms)

Answer 3

(+) color: RED – T. mentagrophytes, T. tonsurans

Question 4

Thiamine Requirement (2)

Answer 4

➢ single most useful nutritional test for dermatophytes
➢ T. tonsurans (+)

Question 5

➢ casein, ammonium nitrate -
➢ 7 agars to determine nutritional requirement of Trichophyton spp.

Answer 5

Trichophyton Agars

Question 6

Growth on Rice grains
➢ sterile non-fortified rice; incubate at _______ for _______
➢ ____ M. canis, M. gypseum; ____M. audouinii

Answer 6

➢ sterile non-fortified rice; incubate at room temp for 10 days
➢ (+) M. canis, M. gypseum; (-) M. audouinii

Question 7

MISCELLANEOUS TESTS FOR YEAST IDENTIFICATION (4)

Answer 7

1. Germ Tube Production
2. Carbohydrate Assimilation
3. CHROM agar Candida
4. Cornmeal Agar

Question 8

most important and easiest to perform for yeast identification

Answer 8

Germ Tube Production

Question 9

Germ Tube Production specimen

Answer 9

uses serum/plasma (fetal bovine serum), expired FFP from blood bank

Question 10

➢ True germ tube – ___________ (__________– can grow at 42degC, __________ – no growth at 42degC))
➢ Pseudogerm tube – ___________ (________)

Answer 10

➢ True germ tube – lacks constriction at base (C. albicans – can grow at 42degC, C. dubliniensis – no growth at 42degC))
➢ Pseudogerm tube – has constriction at base (C. Tropicalis)

Question 11

identifies which carbohydrate a yeast can use aerobically API 20C Yeast ID System: most commonly used

Answer 11

Carbohydrate Assimilation

Question 12

assimilated sugars: wells become turbid with growth

Answer 12

Carbohydrate Assimilation

Question 13

CHROM agar Candida

Answer 13

identifies based on colony colors

Question 14

2nd in importance to sugar assimilation in yeast ID

Answer 14

Cornmeal Agar

Question 15

used to elicit chlamydoconidia production of C. albicans

Answer 15

Cornmeal Agar

Question 16

M27–A3

Answer 16

Broth dilution for yeast testing

Question 17

M38–A2

Answer 17

Broth dilution for mold testing

Question 18

M44–A

Answer 18

Disk diffusion for yeast

Question 19

M51–A

Answer 19

Disk diffusion for mold