2.5.12 Retroviridae

Question 1

Retroviruses contains what enzyme that can turn its DNA to RNA?

Answer 1

Reverse transcriptase

Question 2

What are the 2 viruses associated with Retroviridae?

Answer 2

Oncovirinae
Lentivirinae

Question 3

Associated with malignancies

a. Oncovirinae
b. Lentivirinae

Answer 3

a

Question 4

Examples of Oncovirinae?

Answer 4

HTLV-1
HTLV-2
HTLV-5

Question 5

Tropical spastic parapaersis/ Adult T cell leukemia

a. HTLV-1
b. HTLV-2
c. HTLV-5

Answer 5

a

Question 6

Flower cells on PBS

a. HTLV-1
b. HTLV-2
c. HTLV-5

Answer 6

a

Question 7

Seen in Hairy Cell leukemia

a. HTLV-1
b. HTLV-2
c. HTLV-5

Answer 7

b

Question 8

HIV1 or HTLV 3

a. Oncovirinae
b. Lentivirinae

Answer 8

b

Question 9

What HIV is Prevalent worldwide?

Answer 9

HIV 1

Question 10

HIV is known to be diploid virus

T or F

Answer 10

T

Question 11

Most comm Group for HIV

Answer 11

Group M

Question 12

What Cell is being targeted by HIV?

Answer 12

CD4

Monocytes
Macrophage
Dendritic cells

Question 13

AIDS: If CD4 count is less than ______ cells/ul or presence of opportunistic infections

Answer 13

200

Question 14

Terminal Phase of HIV infection

Answer 14

AIDS

Question 15

Co-receptor for HIV when infecting target host cell?

Answer 15

CXCR4 and CCR5

Question 16

HIV 1
Env:
gag:

Answer 16

HIV 1
Env: gp120 (Transmembrane), gp41 (Docking)

gag: 24 (Capsid), 17 (Matrix)

Question 17

Earlieast marker that we can detect in HIV ?

Answer 17

Genome/Genetic material (HIV RNA)

Question 18

HIV 2
Env:
gag:

Answer 18

HIV 2 : p26, p16, gp125

Question 19

HIV RNA (+) ___ days after infection

Answer 19

10

Question 20

p24 (+) _____ days after HIV RNA infection

Answer 20

4-10

Question 21

Anti HIV persist (+) ______ days after HIV RNA detection

Answer 21

18-38 days

Question 22

Recombinant Viral PRoteins
ex. EIA;Rapid tests

1st generation
2nd generation
3rd generation
4th generation
5th generation

Answer 22

2nd

Question 23

Detetion of Ag and Ab

1st generation
2nd generation
3rd generation
4th generation
5th generation

Answer 23

4

Question 24

Detects and differentiates Antibodies

1st generation
2nd generation
3rd generation
4th generation
5th generation

Answer 24

5

Question 25

Purified Viral lysates as Antigens
Western Blot; IFA

1st generation
2nd generation
3rd generation
4th generation
5th generation

Answer 25

1s

Question 26

Double antigen Sandwich Assay

EIA

1st generation
2nd generation
3rd generation
4th generation
5th generation

Answer 26

3rd

Question 27

Positive for Western Blot?

Answer 27

2 out of 3 bands