Proteins Flashcards

1
Q

Amino acid with sulfur

A

Cys and methionine

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2
Q

Which amino acid can for disulfide bond

A

Cys only

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3
Q

Pk1 pk2 and pkr
pI of acid and base

A

Acid= pk1+pr/2
Base=pk2+pr/2

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4
Q

Histadine side chain

A

Imidazole ring

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5
Q

Basic amino acids

A

Histidine
Lysine
Arginine

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6
Q

Acidic amino acid

A

Aspartic acid
Glutamic acid

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7
Q

Uncharged polar amimo acid

A

Aspargine
Glutamine
Tyrosine

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8
Q

Hydrophobic amino acid

A

Glycine
Alanine

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9
Q

Technique used to synthesis Protein and the dir of protein syn

A

Solid phase
C to N

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10
Q

Most amino acid present in L/D and S/R
With exception

A

Mostly amino acids L and S
Exception cysteine-R

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11
Q

Cleavage site or ctbr

A

C of met

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12
Q

Cleavage site of trypsin

A

Lys and arginine - Cend
K and R

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13
Q

Cleavage site of chymotrypsin

A

Tyrosine, phenylalanine and tryptophan
Y, F, W C end

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14
Q

Cation exchanger, charge and example

A

Charge- negative
Eg

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15
Q

Anion exchanger charge and example

A

Anione ex- Positive
Eg

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16
Q

In Size exclusion chromatography what elute first

A

Large particle

17
Q

Stationary phase if reverse phase chromatography

A

Hydrophobic

18
Q

Alpha gelix can be

A

3.6 10

19
Q

Alpha helix measurements

A

Xyz

20
Q

Beta sheets measurement

A

Dist bbt 2 amino acid- 3.4?

21
Q

Micheal menten eq

A

Vo=Vmax×[S]/Km+[S]

22
Q

Competitive en binds at
, And changes in vmax and Km

A

Active site
Km reduces
vmax same

23
Q

Non competitive binds and effect on Km and Vmax

A

Binds at regulatory site

24
Q

Uncompetitive enzyme binds at, and effect on km and vmax

A

Xyz

25
Q

Mixed inhibitor

A

Xyz

26
Q

Km is

A

Substrate conc at which reaction reached vmax/2

27
Q

Micheal menten graph shape

A

Hyperbolic

28
Q

Linearweer burk plot x and y

A
29
Q

Eddie hoffste plot x and y

A
30
Q

Kcat?

A