glycolysis and pentose phosph

Question 1

how many “priming” or “waterfall” steps are there in glycolysis

Answer 1

3

Question 2

prep phase
step 1: hexokinase

Answer 2

adds phosphate to glucose by cleaving the super high energy beta-gamma phosphoanhydride bond within ATP

Question 3

the beta gamma bond is so high energy in prep phase step 1 that what happens

Answer 3

there is unused free energy even after making the new phosphester bond , released as heat (explaining gibbs free energy)

Question 4

is hexokinase step essentially irreversible

Answer 4

yes

Question 5

T or F
according to le chaterliers principle, G6P never accumulates to a point that causes the reaction to go backwards

Answer 5

t

Question 6

what does step 2 phosphohexose isomerase convert G6P to

Answer 6

Fructose -6-P
this step is governed by levels of substrate

Question 7

whats the most important step in glycolysis?

Answer 7

Phosphofructokinase -1

Question 8

what is the second waterfall step and first committed step for glycolysis

Answer 8

Phosphofructokinase -1

Question 9

whats the fourth step

Answer 9

aldolase splitting the 6 carbon F1,6BP into two 3-carbon molecules, glyceraldehyde 3-
phosphate (GAP) and dihydroxyacetone phosphate (DHAP).

Question 10

is aldolase reaction readily reversible in either direction

Answer 10

yes

Question 11

what can be used for triacylglycerol synthesis (making fatty acids)

Answer 11

DHAP

Question 12

step 5

Answer 12

triose phosphate isomerase

Question 13

only what can proceed to step 6

Answer 13

GAP

Question 14

step 6

Answer 14

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH)

Question 15

what does lactate dehydrogenase oxidize

Answer 15

NADH + H+ in anaerobic conditions