Cell Components Flashcards

1
Q

what microscopy method uses polymers to expand tissue for better resolution

A

expansion microscopy

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2
Q

what is the term for the microscope’s len’s ability to produce separate images of closely positioned objects

A

resolving power

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3
Q

what is a picometer in comparison to a meter

A

one trillionth of a meter

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4
Q

what is a micron in comparison to a meter

A

one millionth of a meter

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5
Q

what is a nanometer in comparison to a meter

A

one billionth of a meter

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6
Q

what consdenser diaphragm setting is the best

A

2/3rd of the objective aperture

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7
Q

what types of cells are MHC class 2

A

macrophages and lymphocytes

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8
Q

what is the major protein of the inner mitochondrial membrane

A

ATP synthase

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9
Q

what are the three digestive enzymes that are integral membrane proteins

A

amylase
pepsin
trypsin

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10
Q

what compartment in the cytoplasm is responsible for sorting and redirecting proteins

A

endosomes

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11
Q

how many membranes do the mitochondria have

A

2

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12
Q

what 2 cells types don’t have mitochondria

A

red blood cells
terminal keratinocytes

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13
Q

what is another name for the centrosome

A

microtubule-organizing center (MTOC)

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14
Q

5 common inclusions that might be found in a cell

A

lipofuscin
hemosiderin
glycogen
crystalline
viral

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15
Q

what two cells typically have glycogen

A

liver and striated muscle

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16
Q

what tests uses fluorescent dyes combines with multiple nucleotide probes to visualize specific genes or portions of genes in a cell

A

fluoresce in situ hybridization (FISH)

17
Q

what are some examples of how FISH testing is used

A

screening for cancer
screening for HIV
prenatal diagnostic testing

18
Q

what is the importance in FISH testing

A

it can test for specific biomarkers in solid and hematological neoplasms

19
Q

what are some advantages of autoradiograph

A

provides high sensitivity and resolution
can be used to analyze the length and number of DNA fragments after they are separated from one another by gel electrophoresis

20
Q

what are some advantages of expansion microscopy

A

chemicals are inexpensive
can be used with ordinary microscopes
superior resolution
expansion capability up to 20 times

21
Q

what is the purpose of anchoring in expansion microscopy

A

reagents bind the proteins or molecules of interest to the probes with fluorescent label

22
Q

what is the purpose of technical homogenization in expansion microscopy

A

the ultrasonic homogenizer and proteinases digests or breaks-down the structural molecules of the cell to prepare the tissue to receive the expander solvent

23
Q

what can cause variances in resolution

A

type of optical system
wavelength
thickness of specimen
fixation
staining intensity

24
Q

what is the method of specimen illumination used for transmitted and reflected light optical microscopy that reduces light source interference

A

Kohler illumination

25
Q

what are some advantages of Kohler illumination

A

even illumination of sample
reduces image artifacts
provides high sample contrast

26
Q

when would you use a phase contrast microscope

A

when examining unstained cells or living cells

27
Q

what is a common microscopic technology used in pathology that transmits over mobile devices uses a slide scanner

A

virtual microscope

28
Q

which structure in the cell makes ribosomes

A

nucleolus

29
Q

what are the three components of the cell membrane

A

lipids
proteins
carbs

30
Q

what are the three purposes of linker proteins

A

provides structure to cells
helps to provide movement to cells
may link cells together

31
Q

what binds mRNA and tRNA

A

ribosomes

32
Q

what component of the cytoskeleton acts as a conveyer belt

A

microtubules

33
Q

what are the three major types of endocytosis

A

pinocytosis
phagocytosis
receptor-mediated endocytosis