PCR

Question 1

What are 3 examples of bioinformatics?

Answer 1

Manage data
retrieve and compare data
data mining & analysis

Question 2

What is added to the end of mRNA to make it a mature mRNA?

Answer 2

Poly-A-tail

Question 3

What is an ORF?

Answer 3

If RNA encodes a protein
comprises a stretch starting with ATG and ends with a stop codon

Question 4

What are stop codons?

Answer 4

TGA
TAG
TAA

Question 5

What is PCR?

Answer 5

exponential amplification of DNA sequences

Question 6

What are 4 applications of PCR?

Answer 6

infectious diseases
forensics
genetic testing
molecular research

Question 7

What are 4 PCR inputs?

Answer 7

Template - DNA containing target
Primers - oligonucleotides flank target
Polymerases - Taq
dNTPs - incorporation into product

Question 8

What are primers?

Answer 8

Short - single stranded oligonucleotide
one pair per target (forward + reverse)

Question 9

What are 6 primer factors to consider?

Answer 9

length
melting temp
annealing temp
GC content
GC clamp
3’ end stability

Question 10

What are 5 primer pair factors?

Answer 10

Amplicon length
product position
Tm of product
optimum annealing temp
primer pair Tm mismatch calculation

Question 11

What are 2 things to avoid in PCR primer design?

Answer 11

Primer secondary structures
cross homology, repeats and runs