Permanent Mounts Flashcards

1
Q

All fungi are gram positive

A

Gram Stain

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2
Q

Crystal Violet + Ammonium Oxalate = _____

A

Hucker Modification

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3
Q

to demonstrate details of fungal elements
It is useful in staining tissue specimens
Detects fungi in tissues

A

Periodic Acid Schiff

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4
Q

Periodic Acid Schiff fungi color

A

pink-red color bright
magenta

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5
Q

Periodic Acid Schiff background color

A

green

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6
Q

detects fungi in histologic sections; brown to black.

A

Gomori Methenamine Silver

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7
Q

Gomori Methenamine Silver outline color

A

black

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8
Q

Gomori Methenamine Silver internal parts color

A

pink black

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9
Q

Gomori Methenamine Silver background color

A

light green

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10
Q

Gomori Methenamine Silver detects

A

Candida
Aspergillus

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11
Q

Demonstrates melanin

A

Gomori Methenamine Silver

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12
Q

detection of Nocardia and Blastomyces dermatitidis

A

Acid Fast (Kinyoun’s)

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13
Q

agent of systemic mycoses

A

Blastomyces dermatitidis

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14
Q

is a branching bacteria, not fungi

A

Nocardia

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15
Q

for blood parasites

A

Giemsa

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16
Q

for bloodstain; used in hematology

A

Wright’s

17
Q

Giemsa or Wright’s stain demonstrates

A

Histoplasma Capsulatum

18
Q

Demonstrates Malassezia furfur
- causative agent of Tinea versicolor, Pityriasis versicolor (an-an)

A

Acridine Orange

19
Q

Detects hyphal pigmentation – to determine if hyaline or dematiaceous

A

Fontana Masson
H&E

20
Q

Acridine Orange fungal element color

A

green fluorescent

21
Q

Acridine Orange epithelial cell color

A

orange

22
Q

non pigmented/lightly
pigmented

A

hyaline

23
Q

darkly pigmented

A

Dematiaceous

24
Q

Demonstrates Cryptococcus neoformans

A

Mayer’s Mucicarmine

25
Q

Gridley’s Stain Hyphae and yeasts color

A

dark blue-pink

26
Q

Gridley’s Stain Tissues

A

deep blue

27
Q

Gridley’s Stain background

A

yellow

28
Q

To detect fluorescence

A

Wood’s Lamp (UVL)