ADSORPTION CHROMATOGRAPHY

Question 1

Adsorption chromatograpy subtypes

Answer 1

1. Thin Layer Chromatography
2. Column Chromatography
3. HPLC
4. Gas Chromatography

Question 2

• SP: solid adsorbent spread thinly on a glass or plastic plate
• Ex: silica gel, alumina CaCO3
• MP: organic solvent which is less polar than the SP
• Ex: hexane, ethyl acetate

Answer 2

Thin Layer Chromatography

Question 3

Used to separate compound from mixture

Answer 3

Column chromatography

Question 4

• SP: solid adsorbent packed in a vertical glass column
• Ex: silica gel, alumina CaCO3
• MP: organic solvent added to the top and flows down through the column
• Ex: hexane, ethyl acetate

Answer 4

Column chromatography

Question 5

• SP: column packed with glass or plastic beads coated with silica derivatized with nonpolar functional group
• MP: liquid pumped through the column with high pressure
• Reverse-phase: water, methanol, acetonitrile
• Normal-phase: hexane, diethyl ether

Answer 5

HPLC

Question 6

• SP: either a solid adsorbent or liquid ion an inert support
• MP: chemically inert carrier gas (helium or nitrogen)

Answer 6

Gas chromatography

Question 7

In column chromatography, the resulting pattern with several bands is called?

Answer 7

Chromatogram

Question 8

In column chromatography, the separation of the sample mixture into a series of narrow bands in a column is is called?

Answer 8

Elution

Question 9

In column chromatography, the fluid entering the column and facilitates separation

Answer 9

Eluent

Question 10

Fluid exiting the column (that is collected in flasks); separated fractions/components

Answer 10

Eluate