Enzyme Kinetics

Question 1

Michaelis menten kinetics: types of reactions

Answer 1

1st order reaction

pseudo 1st order reaction

zero order reaction

Question 2

1st order reaction

pseudo 1st order reaction

zero order reaction

Answer 2

1st order reaction: rate = [S], the concentration of a single substrate is directly proportional to the rate of the rxn. linear relationship between [s] and product formation

pseudo 1st order reaction: only one substrate effects the rxn rate. have multiple substrates but one is already fully saturated the enzyme

zero order reaction: the rate stops increasing even though the [S] is increasing because enzymes are saturated

Question 3

what are the 2 things being measured in michealis-menten equation

Answer 3

vmax= maximum rate
km= enzyme affinity

Question 4

Michaelis-menten equation

Answer 4

Vo= Vmax[s] / ([s]+Km)

Vo= initial velocity, initial rate of rxn. When [s] is high and [p] is low there’s no reverse rxn

Vmax= maximum rate/ velocity

Km= enzyme affinity. indicates how well the enzyme binds a given molecule (inverse relationship)
small km= high affinity
large km= low affinity

Question 5

line weaver-burk

Answer 5

reciprocal of Michaelis menten, straight line graph

Question 6

what are the x intercept and y intercept in line weaver-burk plot

Answer 6

x intercept = -1/Km (NEGATIVE)
y intercept= 1/Vmax

higher Vmax is lower y intercept
higher Km, less affinity = largest x value
lowest Km, highest affinity= most negative X value