Microscopy Flashcards
1
Q
what does the term resolution mean
A
the ability to distinguish two separate objects that are close together
1
Q
what does the term magnification mean
A
the process of enlarging the apparent size of something
2
Q
what does it mean if an image has a high-resolution
A
the higher the resolution the more detail you are able to see
3
Q
what does it mean if an image has low magnification?
A
the low the resolution the less detail you are able to see in the image
4
Q
What is a light microscope?
A
- a relatively cheap easy-to-use microscope which relies on light to produce images.
- has a low resolution of 0.25 micrometres; any details below will be blurry
- ## images are only in white and black
5
Q
what is an electron microscope?
A
- very expensive, hard-to-use microscope which relies on electrons to produce images
- has a high resolution of 0.1 nanocrometres and is able to produce detailed images
- 2000x more magnification than a light microscope.
- view images in colour
6
Q
why is using an electron microscope better than a light microscope?
A
- by using an electron microscope they are able to produce images of high-resolution
- and have a high magnification range which allows them to detect small structures such as mitochondria
7
Q
magnification equation
A
magnification = size of image
———————
size of real object
- 1) measure image size with ruler in mm
- 2) convert mm to nanometres (x1000)
- 3) use the magnification equation to find the magnification
