24. replication and recombination**

Question 1

how is DNA replicated in bacteria?

Answer 1

• a single chromosome replicated in 42 min
  – started from single origin of replication and proceeding around circle bidirectionally

Question 2

how is the process initiated?

Answer 2

• occurs at specific nucleotide sequence
  – at ori
  – when various proteins bind to begin replication
• some proteins that bind to origin of replication are important in making single-stranded regions of DNA accessible for replication
  – chromosomal DNA wrapped around histones/histone-like proteins and is supercoiled