Enzymology

Question 1

Reaction increased as you add substrate

Answer 1

First-order kinetics

Question 2

Substrate concentration reached its maximal value

Answer 2

Saturation Kinetics

Question 3

Reaction rate depends only on enzyme concentration

Answer 3

Zero-Order Kinetics

Question 4

Organic substance that serves as 2nd substrate and is essential to achieve absolute enzymatic acitivity

Answer 4

Coenzymes
-NAD
-NADP

Question 5

Inorganic ions which alter the spatial configuration of the enzymes for proper substrate binding

Answer 5

Activators
- Ca
- Zn
- Mg
- F
- K

Question 6

Inorganic ions attached to a molecule. Examples are catalase and cytochrome oxidase

Answer 6

Metalloenzymes

Question 7

Physically binds to the active site of an enzyme. REVERSIBLE

Answer 7

Competitive inhibitor
- reversible once tinaasan yung concentration ng substrate
- it has the ability to alter the apparent Michaelis-Menten constant

Question 8

Binds an enzyme at a place other than the active site. IRREVERSIBLE

Answer 8

Non-competitive Inhibitor

Question 9

Binds to the ES complex

Answer 9

Uncompetitive inhibitor

Question 10

Enzymes having the same catalytic reactions but slightly different molecular structure

Answer 10

Isoenzymes

Question 11

Enzymes are active at what temp?

Answer 11

25, 30 and 37C

(optimum temp is 37C) - however, enzymes such as kinase may exhibit denaturation at this stage

Question 12

What does temperature coefficient mean (Q10) mean?

Answer 12

It means every 10C increase, there will be twofold increase in enzyme activity

Question 13

can be denature the protein or enzymes

Answer 13

40-50C

Question 14

Temperature near _______may result to inactivation of enzymes

Answer 14

60C to 65C

Question 15

Most physiologic reactions occur in the pH range of

Answer 15

7-8