DNA replication

Question 1

During DNA replication, the two strands separate a number of points, called ____, and each strand can serve as a template upon which the missing partner can be reconstructed by ____ with free nucleotides.

Answer 1

origins of replication

base pairing

Question 2

During DNA replication, nucleotides are brought together by the action of ____ and are hydrogen bonded to the template strand.

Answer 2

DNA - dependent DNA polymerases alpha and delta

Question 3

In the DNA replication of mitochondrial DNA, free nucleotides are brought together by what polymerase?

Answer 3

DNA - dependent DNA polymerase gamma

Question 4

T/F. Replication proceeds in both directions from each initiation point until the two new strands of DNA are complete.

Answer 4

T

Question 5

This method of replication is called _____, as one strand of each new DNA duplex molecule has been conserved.

Answer 5

semiconservative

Question 6

The semiconservative nature of DNA replication can be shown very effectively by growing cells through two cell divisions in culture medium containing _____, an analogue of thymine.

Answer 6

bromodeoxyuridine (BrdU)

Question 7

After growing cells in a culture medium containing bromodeoxyuridine (BrdU), and subsequent staining, ach chromosome will contain one dark (containing the original strand) and one light chromatid, as the strands that incorporate the BrdU stain less intensely. This appearance is known as _____.

Answer 7

harlequin appearance