Lec 5 - Exocytosis and genome recognition Flashcards
what phase in one step growth curve does exocytosis happen
rise period
each virus has avg burst size
how to naked viruses leave cell
once cpasid is made = cell lysis
how do memb bound viruses leave the cell
need to acquire memb
then leave via budding (maybe secondary cell lysis will occur)
what do bacteriophage release to get out of cell
cytolytic molecules
remember - peptidoglycan cell wall more robust than eukaryotic, so cell lysis needs to happen for it to leave
name the primer on poilio that allows RNA replication
VPG
what does polio P1 encode for
capsid subunits
V1 V2 etc
what does polio P2 encode for
protease 2A and toxin 2A
what does polio P3 encode for
replicase
VPG
protease
what does 5’ end of mRNA in cell have that allows ribosomes to recognise
eIF4F complex
how does polio stop the cells protein synthesis
cleaves eIF4F complex with its 2A protease
means cellular RNA cant bind ribosomes
how does polio ensure that the cellular ribosomes can recognise its RNA
has IRES sequence in its genome
which polio protein alter membrane permeability of host
2B toxin
how does influenza virus acquire its membrane
cell’s plasma memb
how does herpes virus acquire its membrane
internal membranes of secretory pathway
what protein does coronavirus use to acquire its secretory membranes
E1
lots deposited in ER