Lec 5 - Exocytosis and genome recognition Flashcards

1
Q

what phase in one step growth curve does exocytosis happen

A

rise period
each virus has avg burst size

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

how to naked viruses leave cell

A

once cpasid is made = cell lysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

how do memb bound viruses leave the cell

A

need to acquire memb
then leave via budding (maybe secondary cell lysis will occur)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

what do bacteriophage release to get out of cell

A

cytolytic molecules
remember - peptidoglycan cell wall more robust than eukaryotic, so cell lysis needs to happen for it to leave

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

name the primer on poilio that allows RNA replication

A

VPG

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

what does polio P1 encode for

A

capsid subunits
V1 V2 etc

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

what does polio P2 encode for

A

protease 2A and toxin 2A

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

what does polio P3 encode for

A

replicase
VPG
protease

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

what does 5’ end of mRNA in cell have that allows ribosomes to recognise

A

eIF4F complex

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

how does polio stop the cells protein synthesis

A

cleaves eIF4F complex with its 2A protease
means cellular RNA cant bind ribosomes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

how does polio ensure that the cellular ribosomes can recognise its RNA

A

has IRES sequence in its genome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

which polio protein alter membrane permeability of host

A

2B toxin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

how does influenza virus acquire its membrane

A

cell’s plasma memb

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

how does herpes virus acquire its membrane

A

internal membranes of secretory pathway

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

what protein does coronavirus use to acquire its secretory membranes

A

E1
lots deposited in ER

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

herpes virus: protein used to get nucleocapsid out of nucleus

A

UL34
UL31
these 2 catalyse budding
and tegument proteins

17
Q

herpes virus: which proteins allow uptake of NC into golgi

A

gE gI and gM
tegument proteins will bind to these and its taken into golgi

18
Q

what needs to be cleaved in HIV to make it infectious

A

Gag polyprotein
(cleaved by HIV protease - used as drug target)

19
Q

what do viruses have that allows it to distinguish viral genomes from cellular gneomes

A

packaging signals

20
Q

packaging signal in SV40

A

sp1

21
Q

packaging signal in herpes virus

A

pac1 and pac2

22
Q

what is a concatemer

A

long strand of DNA, lots of copies of the viral genome

23
Q

influenza has a segmented genome (8 segments) - how does it ensure capsid interacts with correct genomes

A

each segment has unique packaging signal
so its not random (which what was originally thought)