exam 2

Question 1

guanine and cytosine

Answer 1

top

Question 2

adenine and thymine

Answer 2

bottom

Question 3

what way does a DNA sequence read?

Answer 3

3’-5’

Question 4

most restriction enzymes work optimally at human body temperature. What temperature is this?

Answer 4

37 celcius
98.6 fahrenheit

Question 5

DNA forms what structure?

Answer 5

double helix

Question 6

a DNA nucleotide includes what type of sugar?

Answer 6

deoxyribose

Question 7

DNA is composed of

Answer 7

nucleotides

Question 8

define a single nucleotide polymorphism (SNP)

Answer 8

a single base pair of DNA that has a variation of some sort

Question 9

palindrome

Answer 9

reads the same forward and backwards

Question 10

in DNA, the two complimentary strands are held together by what type of bonds?

Answer 10

hydrogen

Question 11

where is the biggest DNA fragment located?

Answer 11

at the top

Question 12

what is the order of the gel bands?

Answer 12

largest to smallest

Question 13

when looking at gel electrophoresis results, how can you tell how many DNA fragments there are

Answer 13

the brighter the band, the more DNA fragments there are

Question 14

how can you determine the protein in a sample?

Answer 14

by determining the amount f DNA in a sample

Question 15

what is an example of a good correlation?

Answer 15

an R^2 value of 0.5

Question 16

what will be used as the independent variable?

Answer 16

distance DNA fragment traveled in cm

Question 17

what will be used as the dependent variable?

Answer 17

DNA fragment size in base pairs

Question 18

what is the relationship between DNA fragment length and rate of migration through a gel?

Answer 18

longer fragments = travel slower

Question 19

what is the relationship between agarose concentration and rate of DNA migration through a gel?

Answer 19

higher agarose concentration = slower travel for DNA fragments

Question 20

what is the name of the enzyme we used in class?

Answer 20

ecoR1