SOLVING 1st exam

Question 1

Field of view equation

Answer 1

Diameter of High Power Field / Diameter of Low Power Field

=

Low Power Magnification / High Power Magnification

Question 2

Field of view conversion: mm to um

Answer 2

1m : 1000 um

Question 3

Line up to 0

Considering this is ___ with 100 divisions , each division is ___ or ____

Answer 3

Eyepiece Micrometer

Stage Micrometer
1 mm
0.01 mm or 10 um

Question 4

Calibration Factor (CF)

Answer 4

Stage Micrometer division subtended by Ocular Micrometer x value of one stage micrometer division (um) /

Ocular Micrometer division subtended by Stage Micrometer

Question 5

Hemaecytometer gen info:

Volume =
Squares used =
mL =
Area = s^2 =
depth =

Answer 5

Volume = 0.004 mm^3
Squares used = 25
mL = 1000 mm^3
Area = s^2 = 0.04 mm^2
depth = 0.01 mm

Question 6

Hemocytometer:

Ave. no of cells in mm^3

ANS:

Answer 6

Average per grid mm^3 / 0.004 mm^3

ANS cells / mm^3

Question 7

Hemocytometer:

Ave. no of cells per cm^3

ANS:

Answer 7

ANS of mm^3/mm3 (1000 mm^3 / 1 cm^3) (10^3)

ANS cells per cm^3

Question 8

Hemocytometer:

Ave. no of cells per mL

ANS:

Answer 8

Ave PER GRID x 25 grids x 10^4 x 10^3

ANS cells/mL

Question 9

Type of microscope used

Answer 9

Compound Light Microscope

Question 10

When counting blood cells, four corner primary squares which has ___ secondary squares which are used for ____

Answer 10

16

leukocytes

Question 11

The three dicisions needed to dilute are called ____

Answer 11

aliquot