monoclonal antibodies - production

Question 1

what is an antibody

Answer 1

a protein made by lymphocyte which bind to certain anitgens because the shapes of the binding sites are complementary to the antigen on the pathogen

Question 2

true of false lymphocytes only produce 1 type of antibody

Answer 2

TRUE

Question 3

what are monoclonal antibodies

Answer 3

cells made from clones of one lymphocyte

Question 4

what is the first step of making monoclonal antibodies

Answer 4

inject a small animal like mouse with a pathogen or antigen

Question 5

what happens to the injected animal

Answer 5

mouse will have immune reaction
making lymphocytes with specific antibody produced to neutralize antigen

Question 6

what is done to the animal (step 3)

Answer 6

harvest the specific lymphocytes from it

Question 7

what happens to lymphocytes once removed from animal

Answer 7

they die quickly

Question 8

what happens to the lymphocytes that are removed

Answer 8

they are fused with cancer cells to produce hybridoma cells

Question 9

why is cancer good in this case

Answer 9

divides uncontrollably to keep lymphocyte alive

Question 10

what happens to the hybridoma cells that produce correct antibodies

Answer 10

isolated in environment to keep creating more of the same