Cell fractination , and centrifugation

Question 1

state and explian the three main steps in cell fractination.

Answer 1

1. Homogenisation: breaks up cells in a tissue sample.
2. Filtration: Removes large debris, and unbroken cells.
3. Ultracentrifugation: Separates out all the cell organelles.

Question 2

Explain why the solution containing the tissue, has to be ice-cold, buffered, and isotonic.

Answer 2

1. Ice-cold: A cold solution that is cold, so that will prevent enzyme activity that breaks down organelles.
2. Buffered solution: This solution keeps the PH constant, so that the digesting enzymes dont get denatured and loose their function.
3. Isotonic: So that we have no unwanted water gain by osmosis, which could cause the cell organelles to burst.

Question 3

what have we done when we broke up the cells in a tissue sample?

Answer 3

We homogeonised it.

Question 4

Why do we filter the water?

Answer 4

In order to remove large debri from the solution.

Question 5

What do we call the solution that has been filtered?

Answer 5

Filtered solution😂

Question 6

Explain the part with the centrifuge

Answer 6

we place the hameogonised, and filtered solutio into a centrifuge. Over there, we filter spin the solution. The higher the speed, the smaller the organlles that are formed at tthe bottom. For example if we spin the centrifuge at sped of 500, the nucleus will fomr down at the bottom.
If we spin the centrifuge at a speed of 2000, the ribosomes will form at the bottom of the solution.

Question 7

What do we call the substance formed at the bottom of the solution.

Answer 7

A pallet.

Question 8

What do we call the remaining solution after the centrifuge?

Answer 8

The supernatant.