White blood cell anatomy and physiology

Question 1

Do wbcs have a nucleus

Answer 1

Yes

Question 2

What colour are wbcs

Answer 2

Colourless

Question 3

They migrate through capillary walls into

Answer 3

Body tissues

Question 4

Granulocytes- polymorphs are types of

Answer 4

Neutrophils
Eosinophils
Basophils

Question 5

Agranulocytes- mononuclear involves

Answer 5

Lymphocytes
Monocytes

Question 6

Romanovsky type stains is a mixture is

Answer 6

A basic dye and an acidic dye- methylene blue and eosin
Used specifically for staining blood smears
Eg. Wrights stain, may grunwald giesma, diff quick stain

Question 7

Proportions of different types of wbc

Answer 7

Differential wbc count
Percent of eavh type of wbc in a blood sample
Perfect varies depending on species

Question 8

Granulopoiesis happens in

Answer 8

Bone marrow

Question 9

Stages of granulopoiesis

Answer 9

1. Myeloblast- first recognisable granulocytic cell
2. Myelocyte- proliferating mitotic pool
3. Metamyelocyte
4. Circulating forms blood

Question 10

Neutrophils- polymorphs features

Answer 10

Approx 12mm diameter
Characteristic segmented nucleus more lobes as the cell matures
Small specific staining granules in cytoplasm

Question 11

What is a marginating neutrophil

Answer 11

Neutrophil still in circulation

Question 12

Neutrophils in the blood are divided into two pools

Answer 12

Circulating
Marginating

Question 13

Extravasation

Answer 13

Neutrophils enter connective tissue by squeezing between endothelial cells lining the blood vessels- diapedesis

Question 14

Functions of neutrophils within the connective tissue

Answer 14

Attracted to site where there is inflammation
Acts as the 1st line of defence against microbial infection
Active phagocyte/ modulate inflammation and tissue damage
2 types of granules

Question 15

When are band neutrophils present

Answer 15

If demand is high, immature forms are released from the BM

Question 16

Horse shoe shaped nucleus is in

Answer 16

Banded neutrophils

Question 17

Eosinophil features

Answer 17

2-8 percent of total wbc
Typically bi lobed nucleus
Characterisation feature- pink cytoplasmic granules.
Eosinophils take 2-6 days to develop in BM
Circulation exit to tissue

Question 18

Eosinophil functions

Answer 18

Important in parasitic infections- degranulate in the presence of helminths
Regulated inflammatory and allergic type reactions
Phagocytosis of antigen-antibody complexes
Deactivate histamine and inhibit the release of histamine and seratonin

Question 19

Basophil features

Answer 19

Rare
Bilobed
Purple- blue granules

Question 20

Basophil function

Answer 20

Role in inflammatory and allergic type reactions
Contain histamine, heparin and seratonin
Supplement action of mast cells

Question 21

Monocytes features

Answer 21

Largest type of wbc
3-8 percent total wbc
Variably shaped nucleus
Cytoplasm- greyish
Develop and stored in BM and spleen

Question 22

Lymphocyte features

Answer 22

Round densely strained nucleus
Sky blue cytoplasm
Size of rbcs

Question 23

Lymphocyte functions

Answer 23

Cell mediated immune reactions
Antibody production
Antigen recognition
B cell lymphopoiesis is completed in the BM