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MT 11 > glasswares > Flashcards

glasswares Flashcards

1
Q

labware can either be

A

glass or plastic

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2
Q

they are more expensive and requires care while cleaning to prevent breakage and scratches

A

glass containers

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3
Q

these containers are not reusable

A

plasticwares

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4
Q

labwares are required to be _____ to eliminate the possibility of contamination of solution due to inadequate cleansing

A

disposable

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5
Q

what are the types of glasswares

A

borosilicate glass (pyrex/kimax)
boron free glassware/ soft glass
corex ( corning)
flint glass
vycor

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6
Q

used for heating and sterilization processes

A

borosilicate glass

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7
Q

characterized by high degreeof thermal resistance

A

borosilicate glass

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8
Q

high resistance to alkaline

A

boron-free glassware

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9
Q

thermal resistance is less compared to borosilicate glass

A

boron-free glassware

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10
Q

made up of alumina-silicate glass that has been strengthened chemically and thermally

A

corex

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11
Q

six times stronger compared to borosilicate glass

A

corex

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12
Q

made up of soda-lime and a mixture of calcium, silicon, sodium oxides

A

flint glass

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13
Q

poor resistance to high temperature

A

flint glass

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14
Q

for solutions that need heating at a high temperature

A

vycor

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15
Q

can be heated to 900 degrees and can be used for extreme high temperature

A

vycor

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16
Q

what are the types of plasticware

A

polyolefins/polyethylene/ polypropylene
polycarbonate
fluorocarbon resins or teflon

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17
Q

chemically inert resins

A

polyolefins

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18
Q

resistant to acids, alkalis, salt solutions

A

polyolefins

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19
Q

has a clear appearance and because of its strength it may be used for centrifuge tube

A

polycarbonate

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20
Q

chemical resistance is less than polyolefins

A

polycarbonate

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21
Q

chemically inert and used for high temperature from -270 degree celcius to +255 degree celcius

A

fluorocarbon resins or teflon

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22
Q

provides non-wettable surface

A

fluorocarbon resins or teflon

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23
Q

used for stir bars and tubing

A

fluorocarbon resins or teflon

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24
Q

what are the common glasswares in the lab?

A

test tubes
pipette
micropipette
automatic pipette
petri dish
beakers
flasks
stirring rod
graduated cylinder
vials

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25
Q

most common glassware

A

test tube

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26
Q

also known as the culture tube (microbiology section) or sample tube

A

test tube

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27
Q

common piece of glassware consisting of a finger-like length of a glass or clear plastic tubing, open at the top and closed at the bottom

A

test tube

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28
Q

used as a container for mixing the specimen of a patient

A

test tube

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29
Q

are glass or plastic tubes that are usually open at both ends

A

pipettes

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30
Q

used to transfer SPECIFIC AMOUNT of liquid from one container to another

A

pipette

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31
Q

used for volumes from 1 to 100 millimeters

A

pipette

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32
Q

one of the skills that medtech should developed inside the laboratory

A

pipetting

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33
Q

what are the classifications for pipet

A

calibration marks or design
drainage characteristics
type

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34
Q

under calibration marks or design

A

to deliver (TD)
to contain (TC)

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35
Q

under drainage characteristics

A

blow-out (TC)
self-drainage (TD)

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36
Q

under type

A

transfer
graduated or measuring pipet

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37
Q

where is calibration mark seen?

A

on the body of the pipette

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38
Q

it DELIVERS THE EXACT AMOUNT it hold into the container

A

to deliver

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39
Q

it HOLDS THE PARTICULAR VOLUME but does not dispense the exact volume

A

to contain

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40
Q

it has a continuous frosted rings (colored rings) on top of the pipet

A

blowout (TC)

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41
Q

exact volume is obtained when the last drop is blown out using an aspirator

A

blowout

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42
Q

absence of frosted rings and liquid can be drain by gravity

A

self draining (TD)

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43
Q

kinds of transfer type

A

volumetric pipet
ostwald folin
van slyke pipet
pasteur pipet

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44
Q

for non-viscous fluid; self-draining; small amount left in the tip should not be blown out; used to deliver a specific volume of liquid

A

volumetric pipet

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45
Q

shaped like rolling pins with a large belly, one blunt end (neck), one tapering end

A

volumetric pipet

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46
Q

for viscous fluid; with etched rings; bulb closer to the delivery tip; these are blown out pipets

A

ostwald folin

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47
Q

bulb is situated between the mouthpiece and the delivery tip

A

van slyke pipets

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48
Q

transfer fluid without consideration of a specific volume

A

pasteur pipet

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49
Q

don’t pay much to graduation marks

A

pasteur pipets

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50
Q

types of graduated or measuring pipet

A

serological pipet and mohr pipet

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51
Q

with graduation up to the tip; blowout pipet

A

serological pipet

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52
Q

without graduation to the tip; calibrated between 2 marks; self draining

A

mohr pipet

53
Q

if the delivery tip is not calibrated up to the 10th mark it is considered a

A

serological pipet

54
Q

if calibration to 10 ml, it is a

A

mohr pipet

55
Q

If a frosted band or two thin rings around the
neck, then it is a

A

blowout pipet

56
Q

these are the manufacturers code for the maximum volume of the pipet

A

thick colored rings or colored dots

57
Q

for quantities less than 1 mL or 1000 microliters; to contain pipets

A

micropipettes

58
Q

examples of micropipettes

A

rbc and wbc thoma pipets
sahli-hellige pipets
lang-levy pipets

59
Q

used for MANUAL CELL COUNTS in the hematology section

A

rbc and wbc thoma pipets

60
Q

manual determination of hemoglobin

A

sahli-hellige pipets

61
Q

the 2 kinds of thomas pipettes

A

rbc pipette and wbc pipette

62
Q

same parts of rbc and wbc pipette

A

calibrated stem
bulb
short stem

63
Q

differences between rbc and wbc pipette

A

bead color
graduation mark
size of the bulb
size of the lumen

64
Q

bead color for rbc thoma pipette

A

red

65
Q

bead color for wbc thoma pipette

A

white

66
Q

graduation mark for rbc thoma pipette

A

graduations up to 101 mark

67
Q

graduation mark for wbc thoma pipette

A

graduations up to 11 mark

68
Q

size of the bulb for rbc thoma pipette

A

larger

69
Q

size of the bulb for wbc thoma pipette

A

smaller

70
Q

size of the lumen for rbc thoma pipette

A

smaller

71
Q

size of the lumen for wbc thoma pipette

A

larger

72
Q

more accurate and precise when it comes in aspirating and dispensing the solution into another container

A

automatic pipettes

73
Q

type of automatic pipettes

A

handheld and automated

74
Q

uses disposable tips and the technologist performs aspiration and dispensing

A

handheld

75
Q

types of automated pipettes

A

air displacement pipette, positive displacement pipette , dispenser or diluter pipette

76
Q

it RELIES TO THE PISTON FOR SUCTION CREATION to draw the sample into a disposable tip

A

air displacement pipet

77
Q

the piston does not come in contact with the liquid

A

air displacement pipet

78
Q

factors that can affect the performance of the air displacement pipet

A

temperature
atmospheric pressure
gravity

79
Q

how does the air displacement pipet works

A
  1. the piston moves to the appropriate position when the volume is set
  2. when the operating button is pressed to the first stop, the piston expels the same volume of air as indicated on the volume setting
  3. after immersing the tip into the liquid, the operating button is released. this will signal for the pipet to aspirate the sample. this creates a partial vacuum, and the specified volume of liquid is aspirated into the tip.
  4. when the operating button is pressed at the first stop again, the air dispenses the liquid. to completely empty the tip, press the operating button to the second stop
80
Q

parts of the air displacement pipet

A

piston
shaft
air space
disposable tip

81
Q

it operates by moving the piston in the pipet tip or barrel, much like a hypodermic syringe

A

positive displacement pipet

82
Q

the piston touches the sample and do not require different tip for each use

A

positive displacement pipet

83
Q

the tip of the piston is disposable

A

positive displacement pipet

84
Q

based on direct contact of the piston with the liquid or sample. the aspirated liquid amount
depends on the

A

dimension of the cylinder or capillary and the movement distance of the piston

85
Q

how does the positive displacement pipet work?

A
  1. the piston moves down inside the tip to make direct contact with the sample
  2. the piston moves up to draw the sample into the tip
  3. when dispensing, the piston descends, and the selected volume is dispensed
86
Q

it obtains liquid from a common reservoir and dispensed repeatedly

A

dispenser or diluter pipet

87
Q

combines sampling and dispensing functions

A

dispenser or diluter pipet

88
Q

parts of a positive displacement pipet

A

shaft
disposable piston
disposable capillary
piston seal

89
Q

they are made primarily of polypropylene

A

plastic tips

90
Q

as the fluid is allowed to drain at the receiving vessel, the pipet should be held in a _________ position with the tip against the side of the receptacle

A

slanted

91
Q

imperfect wetting or the presence of discreet droplet of water indicates that the pipette is not

A

sufficiently clean

92
Q

when we used automatic pipette with tips, make sure that there is no ________ present inside the tip or plastic tip

A

bubbles or spaces

93
Q

petri dish was invented by _______ in _____

A

Julius Richard Petri in 1887

94
Q

simple pair of nesting glass dishes - keeping specimens of growing bacteria sterile

A

petri dish

95
Q

shallow cylindrical containers with fitted lids that are designed specifically for microbiology or culture cell use

A

petri dish

96
Q

typically made of borosilicate glass or clear plastic (usually polystyrene or polycarbonate) and come in variety of sizes

A

petri dish

97
Q

petri dish can’t be reusable or disposable

A

false

98
Q

what is the procedure for glass petri dish?

A

autoclave, remove agar, clean the dish, put it back in the autoclave for sterilization

99
Q

what is the procedure for plastic petri dish?

A

autoclave then discard

100
Q

these beakers can be heated to a higher temperature compared to plastic counterparts, while offering HIGHER CLARITY for CONTENT VISIBILITY and MEASURING

A

glass beakers

101
Q

features of glass beakers

A

uniform sidewalls, bottom thickness with a flat bottom and a beak, or small spout, for easier pouring

102
Q

used for precise volume measurement

A

glass beakers

103
Q

they have _____ on their side that are accurate within 10%

A

graduated markings

104
Q

what are the other term for Erlenmeyer flask

A

also known as conical flask or titration flask

105
Q

features of an erlenmeyer flask

A

flat bottom
conical body
cylindrical neck

106
Q

they have wide bases with sides that taper upwards to a short vertical neck

A

erlenmeyer flask

107
Q

allow the contents of the flask to be mixed by swirling without the risk of spilling, making it suitable for titration

A

slanted size and narrow neck

108
Q

suitable for boiling liquid

A

erlenmeyer flask

109
Q

what is the other term for volumetric flask

A

measuring flask or graduated flask

110
Q

features of a volumetric flask

A

pear-shaped with a flat bottom and made up of glass or plastic

111
Q

they are _____ to contain a precise volume at a certain temperature used for precise dilutions or preparations of standard solution

A

calibrated

112
Q

also known as boiling flask

A

florence flask

113
Q

features of florence flask

A

round body
long neck
often a flat bottom ( sometimes produced with a round bottom and thus need support in order to stand upright)

114
Q

designed for uniform heating, distillation, and ease of swirling
often made of borosilicate glass to resist high temp
designed for chemical resistance

A

florence flask

115
Q

also known as the glass rod

A

stirring rod

116
Q

what are the types of flask

A

erlenmeyer flask
volumetric flask
florence flask

117
Q

use of stirring rod

A

mix chemicals and decanting supernatants

118
Q

stirring rod is made of

A

borosilicate glass or polypropylene plastic

119
Q

usually made of solid glass, about the thickness and slightly longer than a drinking straw, with rounded edges

A

stirring rod or glass rod

120
Q

why is the edge of the stirring rod smooth and rounded?

A

to prevent scratching the surface of glassware during use which may lead to crack when heated

121
Q

also known as the measuring cylinder or mixing cylinder

A

graduated cylinder

122
Q

the graduated cylinder measures the what?

A

volume of a liquid

123
Q

features of a graduated cylinder

A

narrow cylindrical shape with each marked line representing the amount of liquid that has been measured

124
Q

graduated cylinder is usually made up of ?

A

polypropylene for excellent chemical resistance
polymethyl pentene - for transparency
making them lighter and less fragile than glass

125
Q

traditional graduated cylinder is usually narrow and tall to

A

increase the accuracy and precision
of volume measurements

126
Q

is graduated cylinder appropriate to be used in volumetric analysis?

A

nauurrr

127
Q

used for general collection, storage, transportation of samples

A

vials

128
Q

may be sterile, non-sterile, and/or autoclavable

A

vials

MT 11 (4 decks)

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