Red cell antibody detection & ID

Question 1

1. Which of the following factors affects the indirect
   antiglobulin test?
   a. pH
   b. ionic concentration
   c. proportion of antigen to antibody
   d. all of the above

Answer 1

d. all of the above

Question 2

2. Antibody screening cells are of which ABO group?
   a. A
   b. B
   c. O
   d. AB

Answer 2

c. O

Question 3

3. True or false? The antiglobulin test is based on the
   premise that antibodies are globulins.

Answer 3

True

Question 4

4. True or false? Most antibodies require an incubation
   time of approximately 30 minutes at 37°C if saline or albumin test systems are used.

Answer 4

True

Question 5

5. True or false? The FDA places zygosity requirements on
   red cells used for screening in the United States

Answer 5

False

Question 6

6. True or false? Most antibody detection methods used in
   the United States do not include an immediate spin or
   room temperature phase of incubation

Answer 6

True

Question 7

7. Autoadsorption can only be performed if the patient
   has not been recently transfused. Why?

Answer 7

There is a potential that alloantibody would be removed by the autoadsorption.

Question 8

8. If the antibody identified has clinical significance, what
   requirement is placed on any red cells used for transfusion?

Answer 8

The red cells must lack the antigen to which the identified antibody is directed

Question 9

9. What is the hallmark test for paroxysmal cold hemoglobinuria?

Answer 9

Donath-Landsteiner Test