Factors Affecting Enzymes

Question 1

Explain the increase of temperature affecting the enzyme-substrate formed.

Answer 1

1at low temperature there is low kinetic energy given this the rate of reaction is low
2-The increase of Temperature gives kinetic energy to enzymes and substrate to increasing the rate of reaction and the ESCs formed
3-there is an optimum temperature and then the enzyme breaks down the substrate and release it as product another substrate bind to it and one’s the same
And here there is a limit in enzyme or substrate concentration
4-above optimum temperature there will be a denaturing happening and the tertiary structure will be disturbed and the active site shape will change and subtracted can no longer fit and then reaction can’t occur anymore

Question 2

Describe the affect of increasing the ph on enzyme

Answer 2

Enzymes will have optimum ph at which they work effectively in
-a slight change to Ph changes ionisation affecting the binding of substrate to active site,changing its (enzyme)tertiary structure
-at extreme ph levels denaturing occurs and active site is distorted and substrate no longer fits

Question 3

What is Q10?

Answer 3

Temperature coefficient Defines it ,explain the factor by which the rate of reaction increases by 10 degrees.
The rate 2x or 3x every 10 degree increase
At high temperatures denaturing Happens

Question 4

Describe the graph in a non-enzymatic reaction

Answer 4

Increase in reaction velocity is proportional to substrate concentration

Question 5

Explain the graph of enzyme controlled reaction

Answer 5

It is a Fast the reaction but it reaches a point (velocity max)when all the enzyme molecule are occupied and you will need to alter the concentration of enzyme as the active site are occupied in the active site of enzymes

Question 6

How does concentration of substrate and enzyme affect the rate of enzyme controlled reaction.

Answer 6

1-Enzyme-the reaction increases as there is an increase of enzyme concentration this there are more active sites to bind to for substrate.But increasing the concentration won’t affect the rate of reaction as there are active sites than substrate,limiting factor with substrate concentration.

2-as concentration of substrate increases so does the Enzyme-substrate complex but beyond a certain point the rate of reaction don’t increases due to a limiting factor being the enzyme concentration.

Question 7

How does the concentration of inhibitors affects the rate of reaction on an enzyme controlled reaction.

Answer 7

Temporary blocked
As the concentration of inhibitors increases there is a decrease of rate of reaction due to the(competitive) inhibitors binding to active site
And inhibits the substrate to not bind and non competitive bind to allosteric site changing the shape of active site so substrate can bind

Question 8

There are 2 types of inhibitors,what are they?

Answer 8

Reversible inactives an enzyme by non-covalent and irreversible in-activates an enzyme by bonding covalently to particular group of active site
And competitive and non-competitive