SPORE STAIN (BARTHOLOMEW AND MITTWER COLD STAIN)

Question 1

What are the two genera that commonly form endospores? Presence of spores?

Answer 1

Generas:
- Bacillus & Clostridium
Yes for both

Question 2

Under what conditions will sporulation usually occur?

Answer 2

due to harsh environmental conditions

Question 3

What is the primary stain, decolourizer, and counterstain used in the staining of endospores?

Answer 3

Primary stain: Malachite green
Decolourizer : Water
Counterstain : Safranin

Question 4

How does Bacillus and Clostridium can condense into an endospore? (Think about the environmental conditions)

Answer 4

when environmental conditions become too harsh to permit further vegetative growth and reproduction

Question 5

What are the cultures used in spore stain

Answer 5

Bacillus brevis & Clostridium sporogenes