ALAT (GPT) FS* with/without Pyridoxal-5-Phosphate FS (P-5-P)

Question 1

Method???

Answer 1

Optimized UV-test according to IFCC (International Federation of
Clinical Chemistry and Laboratory Medicine) [modified]

…the same with AST

Question 2

Reagents:

R1 ???
R2 ???

Answer 2

R1
✅TRIS (pH 7.15)……. 140 mmol/L
✅L-Alanine …………..700 mmol/L

✅LDH (lactate dehydrogenase)……….. ≥ 2300 U/L

R2
✅2-Oxoglutarate……..85 mmol/L
✅NADH ………………….1 mmol/L

Question 3

Contents of Pyridoxal-5-Phosphate FS ???

Answer 3

✅Good’s buffer (pH 9.6)…………..
100 mmol/L

✅Pyridoxal-5-phosphate……………..13 mmol/L

Question 4

Medications that may cause false
results in patient samples. Blood collection must be performed prior to drug administration.

Answer 4

🐈 Sulfasalazine
🐈 Sulfapyridine

….may cause false
results in patient samples.

Question 5

Reagent stability after mixing…???

Answer 5

6 days……………2 – 8°C
24 hours……….15 - 25°C

Question 6

Specimen stability

Answer 6

3 days at 20 – 25°C
7 days at 4 – 8°C
7 days at –20°C

Question 7

Note:

On assay procedure like…wavelength, temperature, sample/caloibrator, R1, and R2 the same ra with AST…

🐈Also the same on Conversion factor…

Answer 7

🐈🐈🐈

Question 8

Measuring range up to ____ U/L ?
Limit of detection______U/L ?

Answer 8

✅ Measuring range up to 1000 U/L

✅ Limit of detection…..4 U/L

Question 9

Interfering substance…with P-5-P

Answer 9

✅Ascorbic acid ……………30 mg/dL
✅Bilirubin (conju) …..54 mg/dL

✅Bilirubin (unconj)……….54 mg/dL

✅Hemoglobin…………500 mg/dL
✅Lipemia (TAG)………400 mg/dL

Question 10

Interfering substance…WITHOUT P-5-P

Answer 10

✅Ascorbic acid ……………30 mg/dL
✅Bilirubin (conju) …..60 mg/dL

✅Bilirubin (unconj)……….55 mg/dL (sa unconjugated ra na change sa WITHOUT)

✅Hemoglobin…………500 mg/dL
✅Lipemia (TAG)………400 mg/dL

Question 11

In the…..Method comparison (n=154)

🐈Coefficient of correlation ???

Answer 11

0.994