Biotechnological applications in medicine 2 Flashcards
Structure of insulin,gene therapy
Insulin consists of two short polypeptide chains namely?
A and B linked together by disulphide bonds
In which form is insulin synthesized in humans ?
as a pro - hormone
What is the extra stretch in insulin called?
C peptide
what is the main challenge for the production of insulin using rDNA techniques?
getting insulin assembled in a mature form
Which American company prepared two dna sequences of human insulin ?
Eli Lilly , 1983
Where was this insulin introduced into ?
in plasmids of E.coli
Which other human hormone can be made by genetic engg.
somatotropin (growth hormone )
Define Gene Therapy
it is a collection of methods that allows correction of a gene defect that has been diagnosed in a child/embryo.
When was the first clinical gene therapy given
1990
To whom was the first gene therapy given
4 year old girl with Adenosine deaminase deficiency (ADA)
How is ADA caused?
caused due to the deletion of the gene for ADA which causes SCID
SCID stands for ?
Severe Combined Immuno Deficiency
How can ADA be cured ?
1) bone marrow transplantation
2) enzyme replacement therapy
Permanent cure for ADA
If the gene isolates from marrow cells producing ADA is introduced in the cells at early embryonic stage
