Lecture 5 - Microarrays/DNA Sequencing

Question 1

Cloning Definition

Answer 1

Massive Amplification of DNA/RNA sequences

Question 2

Microarray Definition

Answer 2

Set of DNA Sequences representing entire genome arranged in a grid for genetic testing

Question 3

5 types of Arrays

Answer 3

SNP Array
Array CGH
Gene expression profile
Chromatin Immunoprecipitation
Methylation arrays

Question 4

DNA Sequencing Definition

Answer 4

Determination of DNA sequence of nucleotides

Question 5

Features of DNA sequences

Answer 5

Individual genomes are unique except for monozygotic twins
~0.1% variation between individuals

Question 6

Sanger Sequencing Definition

Answer 6

First type of Sequencing
Used in HGP

Question 7

Various names of Second Gen Sequencing

Answer 7

Next Gen Sequencing (NGS)
Massively Parallel Sequencing
Deep Sequencing

Question 8

Second Gen Sequencing General Features

Answer 8

Library Dependent
Library fragments amplified on a solid surface
Reactions take place in parallel
Read shorter fragments

Question 9

2 types of Third Gen Sequencing (TGS)

Answer 9

PacBio SMRT
Oxford Nanopore Technologies

Question 10

2 types of PacBio SMRT

Answer 10

Continuous Long Read
Circular Consensus Sequencing

Question 11

Breadth of Coverage Definition

Answer 11

Amount of genome being covered at a certain depth
Expressed as a percentage