Cell surface reactivity Flashcards

1
Q

what does a cytoplasmic membrane do

A

separates the inside of the cell from the outside - lots of enzymes involved in metabolism reside here

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2
Q

what do bacterial cell walls

A

contain peptidoglycan (polymer of polysaccharides linked by proteins)

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3
Q

what is outside the cell wall

A
  • capsule
  • EPS
  • sheaths
  • S-layers
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4
Q

cell wall + stuff outside the cell =

A

cell envelope

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5
Q

what is EPS (extracellular polymeric substances)

A

mostly made up of polysaccharides, produced inside the cell and excreted through the cell wall

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6
Q

what is EPS the same as

A

the capsule

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7
Q

making EPS requires lots of energy but it is worth it because it can be used as:

A
  1. Protection from desiccation, pH or temperature fluctuations
  2. Adherence to surfaces and stability of the colony
  3. Are an energy and carbon reserve
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8
Q

what is a sheath

A

they act like a sieve, protecting from large molecules - they have a different surface charge than the cell, acting as a mediator between cell and environment

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9
Q

what are sheaths made of

A

methanogens
some cyanobacteria
some Fe- and Mn- oxidisers

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10
Q

what are S-layers

A

structured layers - they are more organised than EPS or sheaths - paracrystalline - they have a similar role to sheaths

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11
Q

what is a functional group

A

specific group of atoms or bonds within organic molecules that are responsible for the characteristic reactions of those molecules

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12
Q

what controls the behaviour of most functional groups

A

pH of environment

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13
Q

what is the phZPC

A

the pH of zero point of charge

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14
Q

what is the phZPC for most bacteria

A

pH 2-4

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15
Q

what happens at low pH

A

surface functional groups are fully protonated, average bacteria surface is neutral or positively charged and hydrophobic

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16
Q

what happens at high pH

A

functional groups are deprotonated, average bacteria surface is negatively charged and hydrophilic (increases with increased pH)

17
Q

most bacteria grow at circumneutral pH meaning…

A

most bacteria surfaces are negatively charged

18
Q

why might cell surfaces with sheaths, S-layers and EPS have more complex charge distributions

A

because these layers can have different compositions and therefore different chrges

19
Q

Because most cells have low pHZPC and therefore mostly negatively charged…

A

they have a tendency to adsorb cations - especially metal cations

20
Q

what are the 3 steps of colonization of surfaces

A
  1. Transport of bacteria to a surface
  2. Initial adhesion via electrostatic attraction
  3. Irreversible attachment via excretion of EPS or appendages