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parasitology lab > Microscope > Flashcards

Microscope Flashcards

1
Q

One lens
Simplest – magnifying glass or pair of reading glasses

A

Simple microscope

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2
Q

Multiple lens

A

Compound microscope

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3
Q

– the power of the microscope to enlarge the image of an object

A

Magnification

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4
Q

– ability of the microscope to distinguish two objects from each other

A

Resolution

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5
Q

PARTS OF MICROSCOPE
Has 2 major parts:

A

Optical parts – magnifies tissue specimens
Mechanical parts – supports the optical parts

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6
Q

Optical parts

A

Ocular lens
Objective lenses

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7
Q

Magnification of ocular lens

A

10X

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8
Q

Red

A

Scanner

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9
Q

Yellow

A

LPO

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10
Q

Blue

A

HPO

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11
Q

B&W

A

OIO

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12
Q

Holds the different objective lenses
It allows the rotation of the lenses while viewing

A

Revolving Nosepiece

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13
Q

Supports the lenses and nosepiece of the microscope and connects to the base of the microscope

A

Arm

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14
Q

Helps in controlling the amount of light that is passing through the opening of the stage

A

Iris diaphragm

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15
Q

Gathers light from the microscope’s light source and concentrate it into a cone of light that illuminates the specimen

A

Condenser

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16
Q

Lower or raise the stage

A

Coarse adjustment knobs

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17
Q

Used for focus on scanning. Usually, the low power lens is used, enabling the movement of the tube.

A

Coarse adjustment knobs

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18
Q

Used for focusing on high power and oil objectives.

A

Fine adjustment knobs

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19
Q

Moves the body tube for focusing when using the high power lens.

A

Fine adjustment knobs

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20
Q

Provides support to help microscope stand upright

A

Base

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21
Q

Primary magnifier of microscope

A

Objective lens

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22
Q

Maintains the correct distance between the ocular and objective lens

A

Ocular tube

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23
Q

Holds the objective lens.
Its rotation helps to change the power of the objective lens

A

Revolving nose piece

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24
Q

Place for holding sample

A

Stage

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25
Q

Keeps the slide with a specimen in place on the stage

A

Stage clips

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26
Q

Structural or Mechanical Components

A

Head
Arm
Base
Turret
Stage/Stage clips
Feedknobs
Coarse and Fine Adjustment Knobs

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27
Q

Regulates or controls the light (contrast)

A

Diaphragm

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28
Q

So that light can reach the specimen

A

Aperture

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29
Q

Holds the specimen slide

A

Stage clip

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30
Q

Directs the light from the source to the specimen

A

Condenser

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31
Q

Light source

A

Illuminator

32
Q

Initial focusing

A

Coarse adjustment knob

33
Q

Fine focusing

A

Fine adjustment knob

34
Q

Adjust the intensity of light

A

Rheostat

35
Q
  • The distance between the two eyepieces (left and right), usually adjustable to fit individual users.
A

Interpupillary distance

36
Q
  • objectives that can be changed with minimal or no refocusing.
A

Parfocal

37
Q

A microscope that is “______” is one which, if it is in focus with one objective, when the objective is rotated, will remain (mostly) in focus.

A

Parfocal

38
Q

If microscope objectives are not_______ it means that you would need to refocus the microscope each time the objective turret is changed to a different magnification.

A

parfocalled

39
Q
  • the object in the center of view will remain in the center when the objective is rotated.
A

Parcentered

40
Q

Alignment

A

Parcentered

41
Q

_______ of a microscope is a function of refractive index.

A

Resolving power

42
Q

The resolution limit of a microscope is the shortest distance between two nearby objects when the images formed by the microscope are properly differentiated.

A

Resolving power

43
Q

Oil and slides have the same ______

A

Refractive index

44
Q
  • the amount of distance required between the objective lens and the top of the object in order for the sample to appear in focus.
A

Working Distance

45
Q

Determined by multiplying the power of the eyepiece (ocular) by the power of the objective lens.

A

Total Magnification Power

46
Q

DO’S and DON’TS (Microscope)

A

Keep the microscope covered with a clean plastic or cloth cover when not in use.

Use a soft cloth dampened with xylene to clean immersion oil from the OlO. Then, polish with a clean lint free cloth.

When preparing the microscope for storage, make sure that the objective lens facing the stage is scanner and the stage is brought down

Manipulate the light intensity adjustment knob to reduce the light emitted by the illuminator to minimum; then, turn off the light switch and unplug.

47
Q

Keep the microscope covered with a______ or ______ when not in use.

A

clean plastic or cloth cover

48
Q

Use a soft cloth dampened with______ to clean immersion oil from the OlO. Then, polish with a clean lint free cloth.

A

xylene

49
Q

When preparing the microscope for storage, make sure that the objective lens facing the stage is_____ and the stage is brought____

A

scanner

down

50
Q

Manipulate the______ to reduce the light emitted by the illuminator to minimum; then, turn off the light switch and unplug.

A

light intensity adjustment knob

51
Q

DO’S and DON’TS (Slides)

A

Reject slides that are:
imperfectly cleaned
surface scratch or notched edge
frosted appearance

Always handle cleaned slides by the edges.

Used slides should initially be placed in water containing a detergent. Then, slides will be cleaned one by one and transferred to a fresh solution of detergent. Rinse then dry with a clean cotton cloth.

Slides are best stored in a dry place.

It is recommended that cleaned slides be stored by 10s.

52
Q

Reject slides that are:

A

imperfectly cleaned
surface scratch or notched edge
frosted appearance

53
Q

Always handle cleaned slides by the_____.

A

edges

54
Q

Used slides should initially be placed in water containing a______. Then, slides will be cleaned one by one and transferred to a fresh solution of detergent. Rinse then dry with a clean cotton cloth.

A

detergent

55
Q

Slides are best stored in a

A

dry place

56
Q

It is recommended that cleaned slides be stored by

A

10s

57
Q

Stool Specimens

Wear:

A

protective safety glasses
gloves
laboratory coat

58
Q

Stool specimen

Use of____________ when needed.

A

biological safety cabinets

59
Q

Stool specimen

DO NOT:

A

eat
drink
smoke
apply cosmetics
manipulate contact lenses in work area

60
Q

Stool specimen

______work surface and after spill or potentially infectious material.

A

Decontaminate

61
Q

Stool specimen

If you have cuts or abrasions on the skin on your hands, cover them with_____ dressing.

A

adhesive

62
Q

Stool specimen

Dispose “sharps” in sharp container for______.

A

decontamination

63
Q

Stool specimen

Remove_____ and wash____ after completing any task

A

gloves

hands

64
Q

Blood Specimens

A

Wear PPE

If you have cuts or abrasions on the skin on your hands, cover them with adhesive dressing.

Use needles and lancets only once and dispose them in “sharp’ container for decontamination.

Remove gloves and wash hands after completing any task

65
Q
  • most commonly used specimen in the diagnosis of parasitic infections
A

Stool

66
Q

Other specimen used for diagnosis of parasitic infections-

A

blood
sputum
urine
body fluids
tissue biopsy

67
Q

Fecal specimen
Collection - need proper instructions and proper containers

A

type of container
size
age of specimen
substances that may interfere with examinations

68
Q

Condenser

Wet mount

A

Lower the condenser

69
Q

Condenser

Stained sample

A

Condenser near the stage

70
Q

Labelling

A

Name of patient
Age
Sex
Time of collection
What specific type of test

71
Q

Type of container

A

Scree type
Tightly sealed
Clean

72
Q

Size of specimen

A

2-3 tbsp

73
Q

Age if soecime

A

1hr ideally

74
Q

Substances that may interfere with examination

A

Antibiotics and many more

75
Q

Stool Fixatives:

A

10% Formalin
LV-PVA
SAF
Schaudinn’s Fixative
Modified PVA Cu or Zn
One-vial Fixatives

parasitology lab (37 decks)

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