Flashcards in 15. Evidence For Relationships Between Organisms Deck (23):
When one species gives rise to another species during evolution, the DNA of the new species...
Will initially be very similar to that of the species that gave rise to it.
How will the DNA of the new species become different from the original species it evolved from?
Due to mutations, the sequences of nucleotide bases in the DNA of the new species will change.
What would we expect to observe as a result of the DNA of any new species changing?
We would expect species that are more closely related to show more similarity in their DNA base sequences than species that are more distantly related.
Name one technique to determine similarities between the DNA of different organisms.
What happens when DNA is heated?
It's double strand separates into it's two complementary single strands.
What happens when you cool two separated strands of DNA which are complementary to each other?
They reform the original double strand.
Describe the process of DNA hybridisation.
-DNA from two species is extracted, purified and cut into short pieces.
-The DNA from one of the species is labelled with a radioactive/fluorescent marker.
- The DNA of both species are mixed together and heated so the strands separate.
- the mixture is cooled to allow the strands to combine with other strands that have a complementary base sequence.
- the temperature is increased in stages, at each stage the degree to which the two strands are still linked together is measured.
- If two species are closely related they will share many complementary nucleotide bases and hence more hydrogen bonds linking them together in the hybrid strand.
- therefore, the higher the temperature at which the hybrid strand splits, the more closely the two species are related.
Explain DNA hybridisation.
Some of the double strands that form will be made up of one strand from each species. This is called hybridisation.
What are the new strands which have been put through hybridisation called?
How can hybrid strands be identified?
These can be identified because they are 50 per cent labelled.
What are the two groups which plants are put into?
Monocotyledons - (single seed leaf, thin, narrow leaves)
Dicotyledons - (two seed leaves, broad leaves)
Base sequences can be...
Compared using computer analysis and phylogenetic tree diagrams. E.g plants at kew.
The sequence of amino acids in proteins is determined by DNA. The degree of similarity in the amino acid sequence of the same protein in two species will therefore reflect how...
Closely related the two species are.
The amino acid sequences of two different species can be...
Compared to see how closely related two or more species are.
How can proteins of different species be compared?
Using immunological techniques.
What is the principle behind comparing proteins using immunological techniques?
Antibodies of one species will respond to specific antigens on proteins such a albumin in the blood serum of another.
How is immunological protein comparison carried out?
-Serum albumin from species A is injected into species B
-Species B produces antibodies specific to all the antigen sites on the albumin from species A
-Serum is extracted from species B; this serum contains antibodies specific to the antigens on the albumin from species A.
- serum from B is mixed with Serum from C
- The antibodies respond to their corresponding antigens on the albumin in the serum of species C.
The response is the formation of a precipitate.
- A and C are being compared here. The role of B is to form antibodies for the serum of A which can combine with antigens in C.
- the greater the number of similar antigens, the more precipitate is formed and the more closely the species are related.
Why is reproduction important to all organisms?
It is the means by which a species can survive over time.
Each individual tries to ensure that...
Their DNA is passed on through the reproductive process, to the next generation.
Courtship behaviour helps to achieve successful mating because:
Recognise members of their own species to produce fertile offspring.
Identify a mate that is capable of breeding, both partners need to be sexually mature, fertile and receptive to mating.
Form a pair bond that will lead to successful mating and raising of offspring.
Synchronise mating so that it is successful.
Why is courtship behaviour used by males?
To determine whether the female is at the receptive stage of her sexual cycle.
When a male courts a female and she responds and gives the male a sign and the process repeats what is this called?