use of vectors

Question 1

what are vectors used for

Answer 1

transfering dna into a cell

Question 2

what are the two types of vectors

Answer 2

invivo
invitro

Question 3

define invivo

Answer 3

transfering the fragment of dna to a host cell using a vector

Question 4

define invitro

Answer 4

using the polymerase chain reaction `

Question 5

what is the vector for dna fragments

Answer 5

bacteria cells take in plasmid dna

Question 6

what needs to happen inorder for bacteria to take in plamid dna

Answer 6

dna fragment needs to be inserted into plasmid

Question 7

how is the plasmid prepared for insersion

Answer 7

dna and plasmid both cut using restriction endonuclease

Question 8

what are the elments of the plasmid and wich are cut

Answer 8

plasmid contains two genes for ristance and during insersion one gene is cut for human dna to enter

Question 9

by cutting the dna what is rleased

Answer 9

the sticky ends from desired protien

Question 10

what then happens to these sticky ends

Answer 10

insterted into plasmid with use of dna ligase

Question 11

what helps to attach dna fragment to plasmid

Answer 11

dna ligase

Question 12

how many cells take up plasmids with dna inside

Answer 12

1%

Question 13

how do we identifie the plasimids with