4.4 Semen (Routine Microscopic Examination) Flashcards
Sperm motility must be performed on well-mixed, undiluted semen ______ upon liquefaction
Immediately
Sperm motility:
S___ and D_____ are both evaluated using approximately __ hpfs
Speed
Direction
20
Sperm motility
No forward progression
a. 4
b. 3
c. 2
d. 1
e. 0
d
Sperm motility
Slow progression, noticeable lateral movement
a. 4
b. 3
c. 2
d. 1
e. 0
c
Sperm motility
Rapid, straight-line motility
a. 4
b. 3
c. 2
d. 1
e. 0
a
Sperm motility
Slower speed, some lateral movement
a. 4
b. 3
c. 2
d. 1
e. 0
b
Sperm motility
No Movement
a. 4
b. 3
c. 2
d. 1
e. 0
e
Normal motility of sperm is More than ______% motile with quality grading of More than or equal to ____ within __ hrs
50
2
1
Abnormal motility may cause by midpiece and tail abnormalities, or male anti sperm antibodies
T or F
T
Sperm concentration and count:
What is the diluent used in formalin, saline or distilled water?
Sodium bicarbonate
Sperm concentration and count:
What is the ratio of the diluent?
1:20
Part of diluent that is use to immobilize sperm
Formalin
NOTE:
Calculation of sperm concentration
- Sperm/mL = average number of sperm counted x 10^5 or 10^6
- Sperm / mL = (Average no. of sperm counted x Dilution x 1000) / (No. of squares counted x volume of each square)
- Sperm/ejaculate = (sperm/mL) x Specimen volume
Normal values for sperm concentration?
> 20 Mil/mL
Sperm count is by counting in 2 WBC sq. or ___ RBC sq per side
5
