Yeast/C. Elegans

Question 1

replicative lifespan

Answer 1

number of cell divisions a mother cell can undergo

Question 2

what indicates replicative age?

Answer 2

of bud scars

Question 3

how does magnetic sorting work

Answer 3

biotin binds to cell wall
wash + add to new culture
stravidin binds to biotin coated iron beads
pour out + expose to magnet

Question 4

why does magnetic sorting work (yeast)

Answer 4

daughters do not inherit their cell walls from their mothers

Question 5

what contributes to replicative aging

Answer 5

asymmetric distribution

Question 6

mito-ROGFP

Answer 6

reports relative oxidation in the matrix

Question 7

how to report relative oxidation

Answer 7

look at the ratio of 2 excitation maxes

Question 8

which type of mitochondria are delivered to the bud

Answer 8

higher quality, less oxidized

Question 9

what enables normal daughter replicative lifespan

Answer 9

asymmetric distribution of aging factors

Question 10

ERCS (Extrachromosomal rDNA) …

Answer 10

titrates essential rep factors
indicated genome instability

Question 11

chronological lifespan

Answer 11

length of time that a non dividing yeast cell survives

Question 12

mutations in TOR (kinase)

Answer 12

increase chronological lifespan of yeast

Question 13

first male then female

Answer 13

c. elegans

Question 14

which tissue do c. elegans model aging in

Answer 14

non-mitotic

Question 15

do c. elegans replace their cells

Answer 15

no

Question 16

when are somatic cell divisions complete in c. elegans

Answer 16

larval development

Question 17

cameleon

Answer 17

genetic tool for measuring change in Ca2+

Question 18

brainbow

Answer 18

neuron color coding, can pair w/ Ca2+ activity

Question 19

how to induce a random change in dna

Answer 19

chemical

Question 20

how to induce site-directed change in dna

Answer 20

crispr

Question 21

guide RNA (crispr)

Answer 21

matches piece of DNA to modify

Question 22

CAS9

Answer 22

cuts DNA

Question 23

RNAI functional knockout

Answer 23

dsRNA produced in vitro induces degradation of corresponding mRNA

Question 24

phenocopy

Answer 24

looks like phenotype of a gene mutation, but only the transcript is degraded

Question 25

genetic screen strategy

Answer 25

mutagenize, screen, map, clone

Question 26

what characterizes aging in worms

Answer 26

decrease in movement

Question 27

“left on” gene expression…

Answer 27

contributes to age-related decline

Question 28

evidence for stochastic aging

Answer 28

mutations in GFP reporter can preserve muscle integrity, extending lifespan

Question 29

c-elegans experience…

Answer 29

human like sarcopenia

Question 30

what cell nuclei changes in animals during aging

Answer 30

muscle cells