science dna technology

Question 1

dna technology

Answer 1

the wide range of techniques used to study, improve, or manipulate genetic materials

Question 2

dna fingerprinting

Answer 2

isolating dna from an unknown sample to be identified with known sample

Question 3

gene isolation

Answer 3

getting location of specific gene

Question 4

restriction enyme

Answer 4

used in biotechnology to cut dna

Question 5

gene gun

Answer 5

transfer or blast into target molecule

Question 6

gel electrophoresis

Answer 6

chemical separation procedure used to isolate dna fragments

Question 7

vector/plasmid

Answer 7

circular dnas in bacteria

Question 8

electrophoresis lab technique

Answer 8

separates dna, or dna, or protein molecules based on their size and electrical charge

Question 9

polymerase chain reaction

Answer 9

make millions of copies of a small segment of dna

Question 10

gene amplification

Answer 10

results from gene duplication

Question 11

probe

Answer 11

single stranded dna fragment that is a complement of the two complementary strands that make up the gene.

(if separated, radioactive gene probe attaches to its complement in the open strand)

Question 12

gene identification

Answer 12

determines which of the dna fragments isolated is the correct one for insulin production

may be done bye
- screening for the protein product
- screening for the gene using probe

Question 13

dna sequencing

Answer 13

studying the nitrogen base sequence of cells (insulin gene)
- used to determine nucleotide sequence

Question 14

gene mapping

Answer 14

finding the exact location of gene in the 46 chromosmes

Question 15

recombinant dna technology

Answer 15

gene to vector
- process of creating dna molecule by joining together dna or segments of dna obtained from two different species
- introducing insulin gene to bacterium

Question 16

gene cloning

Answer 16

multiple copies of desired gene are produced

Question 17

transgenics

Answer 17

genetically modified organisms
- produced through recombinant dna technology

Question 18

practical applications of dna technology

Answer 18

medicine
newborn screening (done on infants to find out if they have diseases)
immunization (nonpathogenic virus used as vaccine)
gene therapy (correct genetic disorders. extract cells from patient, addition of functional genes)

Question 19

enuncleation

Answer 19

getting nucleus of egg cell from donor