B14 - Variation & Evolution

Question 1

What is variation?

Answer 1

Differences in the characteristics of individuals in a population is called variation.

Question 2

What are the causes of variation within a species?

Answer 2

● Genetics
● Environment
● A mixture of both of the above

Question 3

What is genetic variation?

Answer 3

● Variations in the genotypes of organisms of the same species due to the presence of different alleles.
● Creates differences in phenotypes.

Question 4

What creates genetic variation in a species?

Answer 4

● Spontaneous mutations
● Sexual reproduction

Question 5

What is a mutation?

Answer 5

A random change to the base sequence in DNA which results in genetic variants. They occur continuously.

Question 6

State the three types of gene mutation

Answer 6

● Insertion
● Deletion
● Substitution

Question 7

How may a gene mutation affect an organism’s phenotype? (3)

Answer 7

● Neutral mutation does not change the sequence of amino acids. Protein structure and function same. No effect on phenotype.
● Mutation may cause a minor change in an organism’s phenotype e.g. change in eye colour.
● Mutation may completely change the sequence of amino acids. This may result in a non-functional protein. Severe changes to phenotype.

Question 8

What is the consequence of a new phenotype caused by a mutation being suited to an environmental change?

Answer 8

There will be a rapid change in the species.

Question 9

What is evolution?

Answer 9

● A gradual change in the inherited traits within a population over time.
● Occurs due to natural selection which may result in the formation of a new species.

Question 10

Outline the theory of natural selection

Answer 10

All species of living things have evolved from simple life forms that first developed more than 3 billion years ago.

1. Genetic variation exists due to spontaneous mutations.
2. Selection pressures (e.g. competition, disease) exist.
3. Random mutation gives an organism a selective advantage.
4. Organism is better adapted to the environment and survives.
5. Organism reproduces, passing on its beneficial alleles.
6. Frequency of advantageous alleles increase.

Question 11

How do two populations become different species?

Answer 11

When their phenotypes become different to the extent that they can no longer interbreed to produce fertile offspring.

Question 12

What is selective breeding?

Answer 12

The process by which humans artificially select organisms with desirable characteristics and breed them to produce offspring with similar phenotypes.

Question 13

Outline the main steps involved in selective breeding

Answer 13

1. Identify a desired characteristic e.g. disease resistance.
2. Select parent organisms that show the desired traits and
   breed them together.
3. Select offspring with the desired traits and breed them together.
4. Process repeated until all offspring have the desired traits.

Question 14

Give examples of characteristics selected for in selective breeding.

Answer 14

● Disease resistance in crops
● Higher milk or meat production in animals
● Gentle nature in domestic dogs
● Large flowers

Question 15

What are the main advantages of selective breeding?

Answer 15

Creates organisms with desirable features:
● Crops produce a higher yield of grain
● Cows produce a greater supply of milk
● Plants produce larger fruit
● Domesticated animals

Question 16

Other than in agriculture, where else is selective breeding useful?

Answer 16

● In medical research
● In sports e.g. horse racing

Question 17

Outline the disadvantages of selective breeding (4)

Answer 17

● Reduction in the gene pool (which becomes especially harmful if sudden environmental change occurs).
● Inbreeding results in genetic disorders.
● Development of other physical problems e.g. respiratory problems in bulldogs.
● Potential to unknowingly select harmful recessive alleles.

Question 18

What is genetic engineering?

Answer 18

● The modification of the genome of an organism by the insertion of a desired gene from another organism - genes from chromosomes of humans and other organisms can be ‘cut out’ and transferred to cells of other organisms.
● Enables the formation of an organism with beneficial characteristics.

Question 19

Give an example of uses for genetically modified plants.

Answer 19

● Disease resistance
● Produce larger fruits

Question 20

What is a use for genetically modified bacteria cells?

Answer 20

To produce human insulin to treat diabetes mellitus.

Question 21

Describe the benefits of genetic engineering (3)

Answer 21

● Increased crop yields for growing population e.g. herbicide-resistance, disease-resistance.
● Useful in medicine e.g. insulin-producing bacteria, anti-thrombin in goat milk, possibility to overcome some inherited disorders (being explored in medical research).
● GM crops produce scarce resources e.g. GM golden rice produces beta-carotene (source of vitamin A in the body).

Question 22

Describe the risks of genetic engineering (4)

Answer 22

● Long-term effects of consumption of GM crops unknown.
● Negative environmental impacts e.g. reduction in biodiversity, impact on food chain, contamination of non-GM crops forming ‘superweeds’.
● Late-onset health problems in GM animals.
● GM seeds are expensive. LEDCs may be unable to afford them or may become dependent on businesses that sell them.

Question 23

What is the name for crops that have had their genes modified?

Answer 23

Genetically modified (GM) crops e.g. those modified to be resistant to insect attack and herbicides.

Question 24

What is Bacillus thuringiensis (Bt)?

Answer 24

● Insect larvae are harmful to crops.
● Bt is a bacterium which secretes a toxin that kills insect larvae.

Question 25

How is genetic engineering used to protect crops against insects?

Answer 25

● The gene for toxin production in Bt can be isolated and inserted into the DNA of crops.
● Bt crops now secrete the toxin which kills any insect larvae that feed on it.

Question 26

What are the benefits of Bt crops? (3)

Answer 26

● Increased crop yields (fewer crops damaged).
● Lessens the need for artificial insecticides.
● Bt toxin is specific to certain insect larvae so is not harmful to other organisms that ingest it.

Question 27

What are the risks of Bt crops? (3)

Answer 27

● Long term effects of consumption of Bt crops unknown.
● Insect larvae may become resistant to the Bt toxin.
● Killing insect larvae reduces biodiversity.

Question 28

Describe the process of genetic engineering

Answer 28

1. DNA is cut at specific base sequences by restriction enzymes to
   create sticky ends.
2. Vector DNA cut using the same restriction enzymes to create
   complementary sticky ends.
3. Ligase enzymes join the sticky ends of the DNA and vector DNA
   forming recombinant DNA.
4. Recombinant DNA mixed with and ‘taken up’ by target cells.

Question 29

What is a vector?

Answer 29

A structure that delivers the desired gene into the recipient cell e.g. plasmids, viruses.

Question 30

How can plants be cloned? (2)

Answer 30

● Taking plant cuttings
● Tissue culture

Question 31

What is tissue culture?

Answer 31

Using small groups of cells from part of a plant to grow identical new plants.

Question 32

Describe how plants are grown using tissue culture

Answer 32

1. Select a plant that shows desired characteristics.
2. Cut multiple small sample pieces from meristem tissue.
3. Grow in a petri dish containing growth medium.
4. Transfer to compost for further growth.

Question 33

What must be ensured when preparing tissue cultures?

Answer 33

Ensure aseptic conditions to prevent contamination by microorganisms.

Question 34

What does the growth medium contain?

Answer 34

Nutrients and growth hormones

Question 35

What are the advantages of growing plants by tissue culture? (4)

Answer 35

● Fast and simple process.
● Requires little space.
● Enables the growth of many plant clones with the same desirable characteristics.
● Useful in the preservation of endangered plant species.

Question 36

What are the disadvantages of growing plants by tissue culture? (4)

Answer 36

● Reduction in the gene pool.
● Plant clones often have a low survival rate.
● Could unknowingly increase the presence of harmful recessive alleles.

Question 37

Give a brief overview of the plant cuttings method of plant cloning

Answer 37

• Older but simpler method than tissue culture.
• Gardeners use this method to produce many identical new plants from a parent plant.

Question 38

Detail the process of the plant cuttings method of plant cloning

Answer 38

1. A branch is cut off from the parent plant.
2. The lower leaves of the branch are removed and the stem is
   planted.
3. Plant hormones are used to encourage new root development.
4. A plastic bag is used to cover the new plant to keep it warm and
   moist.
5. New roots and a new plant is formed after a few weeks.

Question 39

What does embryo transplanting involve?

Answer 39

● Pre-specialised cells from a developing animal embryo are split apart.
● The resulting separate but identical embryos are transplanted into host mothers.

Question 40

Describe how adult cell cloning is performed

Answer 40

1. The nucleus is removed from an unfertilised egg cell.
2. The nucleus from an adult body cell, such as a skin cell, is inserted into the egg cell.
3. An electric shock stimulates the nucleated egg cell to divide and it forms an embryo.
4. The embryo cells contain the same genetic information as the adult body cell.
5. When the embryo is a ball of cells, it is inserted into the uterus of an adult female to continue developing.