DETECTION AND IDENTIFICATION OF MICROORGANISMS Flashcards
Specimen transport systems:
Sterile containers
Sterile cups,
screw-capped tubes,
stoppered tubes,
Petri dishes
Specimen transport systems:
Swabs
Calcium alginate swabs,
Dacron swabs,
cotton swabs,
nasopharyngeal-urogenital swabs,
Swab Transport System
Specimen transport systems:
Specialty system
N. gonorrhoeae transport systems, SETS.
Specimen transport systems:
Proprietary systems
Molecular testing,
Neisseria gonorrhoeae
transport systems,
STAR buffer42
Specimen transport systems:
Anaerobic transport systems
Starplex Anaerobic Transport system (Fisher),
BBL Vacutainer Anaerobic
Specimen Collector
Specimen transport systems:
Viral transport systems
BD Cellmatics Viral Transport Pack,
BBL Viral Culturette
would determine the functionality of the assay .
Positive controls
used to monitor any carry-over contamination.
Reagent blank or contamination control
would determine if the assay or sample is contaminated or not.
Negative controls
are used to rule out false-negatives due to amplification failure. These
controls are usually housekeeping genes such as B-actin which are always present in a human sample.
Amplification controls
are amplification controls that monitor paricular steps of an amplification method
Internal controls
The following are the types of Internal control.
Homologous extrinsic control
Heterologous extrinsic control
Heterologous intrinsic control
Extensive validation must be demostrated before establishing a new molecular-based test. What must be determinef?
The sensitivity, specificity and reproducibility of the assay must be determined.
Quality Control
False-positives:
- Presence of dead or dying microorganisms in the sample of a patient taking antimicrobial agents.
- Contamination
Quality Control
False-Negatives:
- Organisms may be present, but the nucleic acid was degraded during collection, transport, and or extraction.
- Amplification proceures are inhibited.
