Lab 2: Microscopy Flashcards
Function of the Ocular Lens
Magnifies the image of the specimen produced by the objective lens.
Function of the Revolving Nose Piece
Holds and positions objective lenses, rotates to switch between them.
Function of the Stage Clips
Holds the specimen slide in place securely.
Function of the Iris Diaphragm
Controls amount of light passing through the condenser.
Function of the Condenser Lens
Focuses and controls the intensity of the light passing through the specimen
Function of the Coarse Adjustment
Moves the stage up and down to focus the specimen.
Function of the Fine Adjustment
Makes small adjustments to fine-tune focus.
Function of the Objective Lens
Further magnifies the image before it reaches the eyepiece.
Function of the Stage
The flat surface for your slide to rest on.
Function of the Mechanical Stage Knob.
Bigger knob, moves your slide from left to right.
Function of the Mechanical Stage Adjustment.
Smaller knob, moves your slide up and down.
Why is the microscope important for studying biological specimens?
The microscope allows us to visualize very small particles and structures of the cell in great detail.
As magnification increases…
Size of the image ____________.
Intensity of light ____________.
Field of view ____________.
Depth of field __________.
Resolution __________.
Size of the image increases.
Intensity of light decreases.
Field of view increases.
Depth of field decreases.
Resolution has no change.
Advantages/Disadvantages of observing a specimen at lower power vs higher power.
At lower power, there is more light and a greater field of view but lower resolution. At higher power, there is higher resolution but less light and a lesser field of view.
Formula for calculating the diameter of the field of view at other magnifications.
Unit conversions
