L26: Blood Flukes Flashcards

1
Q

What does the digestive tract of Trematodes consist of

A

fore gut and two cacea

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2
Q

Go in depth about whats in the foregut of treamtodes

A
  • anterior mouth, pharynx, esophagus
  • ingestion and assimilation
    *The mouth surrounded by a muscular oral sucker for attachment and feeding
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3
Q

Go in depth about whats in the two caeca of treamtodes

A
  • longitudinal blind tubes
    ✓ intestine-like
    ✓ variable length depending on trematode species
    ✓ Digestion and absorption of food occur in the caeca
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4
Q

What is the tegument in Trematodes

A

syncytium – a multinucleated tissue with no cell boundaries

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5
Q

What is the synticum made of

A
  • outer zone – the distal cytoplasm which is bound by the
    plasma membrane
  • An inner zone – the proximal cytoplasm
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6
Q

What is inner/outer zone of syncytium. What are they composed of

A

proximal cytoplasm / distal cytoplasm

Distal
* glycocalyx is a surface coat covering the entire plasma
membrane
* Surface invaginations function much like microvilli
* The glycocalyx and surface invaginations same
function as in cestodes
* Tegumental spines embedded in the distal cytoplasm and
project outward from the surface

Proximal
*It is connected to the distal cytoplasm by cytoplasmic bridges
* Contains nuclei, endoplasmic reticulum, Golgi complexes and
mitochondria
* involved in repair and maintenance of components of the
distal cytoplasm

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7
Q

Whare the zones of the muscular system. Other key muscle

A

subtegumental:
underlying tegumes of longi, circular and diagnotl layers

gastodermal
line cacare to help move food down

ventral scucker: contractile muscle fibres located mid verntrally for attachment, no opening here

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8
Q

What are general characteristrics of somoregulatory system

A

consits of protenephridia, a tubular system without opening made of flame cells

flame cells has flagella, remove excess water by creating hydrostatic pressure

flame cell open to terminal tubule tat converge from larger tubes leading to excretory bladder

duct of bladder opens to exterior of organism located posterioly

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9
Q

How can trematodes reproduce

A

✓ Self-fertilization in hermaphroditic worms
✓ Cross-fertilization in hermaphroditic worms
✓ Mating between 2 dioecious worms – this occurs ONLY in Schistosoma spp

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10
Q

Describe the male reproductive system

A
  • Generally, includes 2 testes, 2 vas efferens, a common vas deferens, a cirrus, cirrus sac, seminal vesicle and the prostate gland
  • Depending on the species, one or more of these components may be missing, in different size, or in a different position
  • Schistosomes are multi-testicular
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11
Q

Describe the female reproductive system

A

Consists of a single ovary that forms ova
* Depending on the species, the ovary may be anterior/ posterior to, or between the testes

  • Other components are the oviduct which comprises the ootype (where fertilization occurs), Mehli’s gland and vitelline glands
    (both function to secrete the eggshell), uterus (a long, convoluted tube) and Laurer’s canal (in some species)
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12
Q

Draw the reproductive sytem

A

Male reproductive system of a digenetic trematode

Female reproductive system of a digenetic trematode

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13
Q

Describe trematode eggs

A

Eggs are ovoid, shelled and contain the embryo and other materials

  • The typical eggshell is equipped at one end with a lid-like
    structure, the operculum
    ✓ The operculum allows the larva to hatch
  • In species lacking an operculum (mainly schistosomes), the
    eggshells rupture longitudinally when larva (miracidium) hatch
  • Hatching occurs only under precise conditions of temperature,
    osmolarity and light
  • Specific size and structural characteristics of trematode eggs are
    useful in diagnosis
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14
Q

What time of parasite are trematodes

A

obligate parasite

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15
Q

In general describe trematode life cycle

A

✓ 1st intermediate host is an invertebrate, almost always a mollusk (harbors larval stages)

✓ 2nd intermediate host includes fish/ arthropod/ another mollusk (harbors the encysted juvenile trematode)

✓ Definitive host is a vertebrate (harbors the adult trematodes)

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16
Q

What are the life stages of trematodes, describe them

A
  1. Miracidium – The 1st larval form that hatches from the egg. In most species it is ciliated and infects the 1st intermediate host
  2. Sporocyst – Metamorphoses from miracidium following loss of the cilia and other organelles
  3. Redia – The 2nd larval form that develops in the 1st intermediate host. This stage is absent in some species
  4. Cercaria – The 3rd larval form. It also develops in- & emerges from the 1st intermediate host.
  5. Mesocercaria – Forms in the 2nd intermediate host but is only present when there is a 3rd intermediate host
  6. Metacercaria – Forms in the 2nd intermediate host, when present in the life cycle. It must be ingested by the definitive host
17
Q

General characteristics of Schistosoma

A

blood flukes infect humans and animals

cause schistomiasis aka snail fever aka bilharziasis

18
Q

How are schistosoma unique

A

✓ Their life cycle involves one intermediate host and one definitive host

✓ Cercaria infect the definitive host directly, by penetrating the body surface rather than by being ingested

✓ Adult worms parasitize the intravascular niche

✓ They are dioecious and sexually dimorphic i.e., male and female reproductive organs are in separate worms

19
Q

Schistosoma male and female worms describe

A

The adult male is robust and possesses a ventral groove called the gynaecophoral canal

✓ The female worm is held in the gynaecophoral canal, permitting almost continuous mating

✓ They possess 5 to 9 testes

✓ The male genital pore opens ventrally, posterior to the ventral sucker

✓ Males lack a cirrus

female: smaller, slender, position of ovary depends on species

20
Q

Life Cycle of Schistosoma Species

A

Schistosoma eggs are eliminated with the feces or urine, depending on
species
2. under appropriate conditions, the eggs hatch and release miracidia
3. Miracidia swim and penetrate specific snail intermediate hosts
4. In the snail, miracidia first develop through two generations of sporocysts
5. Cercaria are produced from the second generation of sporocysts, and these
are released from the snail into water
6. The infective cercariae swim, and penetrate the skin of the human host
7. Within the definitive human host, cercariae shed their forked tails, becoming
schistosomulae
8. Schistosomulae migrate via venous circulation to the lungs, then to the heart
9. They then migrate to, and develop in the liver, exiting the liver via the portal
vein system when mature
10. Male and female adult worms copulate and reside in the mesenteric venules.
S. japonicum is frequently found in the veins of the small intestine (A), while
S. mansoni occurs more often in the veins of the large intestine (B). Most
often, S. haematobium inhabits the veins of the bladder (C). Females deposit
eggs in the venules. Eggs move toward the lumen of the intestine (S. mansoni
and S. japonicum) and of the bladder (S. haematobium).
11. The eggs are eliminated with feces or urine respectively

21
Q

What schistoma species are of medical importance

A

S. haematobium
S. manosoni
S. japonicum

22
Q

Key characteristics of relevant schistomas species

A

S. haem:
humans are definitve, urinary, f drop 30 eggs deposited single

S.mansoni
humans are definitive, infects primates, intestinal schistom, 190-300 eggs deposited single

S.japo
many mammal definitive hosts, zoonotice, humans can become definite, most pathogenic, 3500 eggs deposited in clusters

23
Q

Clinical presentation

A

stage 1:
localised dermatisits, self limited

stage 2:
katayma syndone, delayed hypersneisitve cause schistomule moves, toxic reaction, pulomonrary congestion, eosinophilia, self limited

stage 3
2 months-yrs after infection, due to granula formation around eggs
granulomas maybe be ectopic, blood stool, dysentery, hepatosplenomegaly and pulmonary hypertension, fatal

24
Q

How does immune system evade schistosomes

A

molecular mimicry

Utilization of an immune resistant outer tegument
✓ Membrane alteration by coating the parasite tegument with host proteins

✓ The secretion of several potent proteases

✓ Production of specific immunomodulatory factors affecting immune cell functions

✓ Granuloma formation

25
Q

Epidemiology of Schist

A

poor, rural
lack of hygine, agricutural, reacrational, near snails, using water for domestic things, eco turism

Africa, middle east for haem and mansoni

japo is in indo and philpipins

26
Q

Schisto diagnosis

A

eggs in stool/urine
rectal biopsises, bladder/mucoal biopsy
immunodiagonostic for travellers

27
Q

Schisto treatment

A

praziquantel disrupt integrity of teguments, expose paraity to host antibodies
antihistamines
antibioitics for secondary infection

28
Q

Cercarial dermatitis

A

swimmers itch by schisto im aquatic birds/mammals
clinically distinct from schisto cause not caused by human shistomsomes

cause unnatural host cercarieae don’t go to blood stream but go to skin and destroyed by ost immune

allergernic substances releases => locallised inflammatory reaction

worldwide issue